2种海洋微藻对氮浓度生长响应的研究  

Growth Response of Two Marine Microalgae to Nitrogen Concentration

作　　者：蔡卓平[1,2] 肖群[1] 黄伟伟[1] 段舜山[1] 

机构地区：[1]暨南大学水生生物研究中心,广东广州510632 [2]中国科学院南海海洋研究所,广东广州510301

出　　处：《水产科学》2010年第11期629-633,共5页Fisheries Science

基　　金：国家自然科学基金资助项目(40876074)

摘　　要：以中肋骨条藻和三角褐指藻为试验材料,探讨2种海洋微藻对氮浓度的生长适应性差异。结果发现2种海洋微藻的生长速率显著受氮浓度的影响。试验结束时,氮质量浓度为0.75、7.5、75 mg/L和150 mg/L时,中肋骨条藻的叶绿素荧光值分别为139.5、816.3、881.9μg/L和868.5μg/L;三角褐指藻的叶绿素荧光值分别为54.3、572.9、974.4μg/L和976.4μg/L,说明高质量浓度氮促进海洋微藻增殖,但超出一定的氮质量浓度范围,促进作用不明显。2种海洋微藻对氮质量浓度的生长响应存在显著差异,中肋骨条藻比三角褐指藻更忍耐低氮质量浓度的胁迫。结果证实氮营养在海洋微藻生长繁殖过程中的重要性,不同微藻对氮营养的生长适应性差异是决定微藻在海洋环境中占据优势程度的一个重要因素。The growth patterns of two marine diatoms,Skeletonema costatum and Phaeodactylum tricornutum,were investigated under nitrogen concentrations of 0.75,7.5,75 and 150 mg/L by chlorophyll fluorescence determination.The growth rates of both algae were found to be significantly affected by nitrogen concentrations.In the experiments,chlorophyll fluorescences of S.costatum were 139.5,816.3,881.9 μg/L and 868.5 μg/L under nitrogen levels of 0.75,7.5,75 mg/L and 150 mg/L,respectively,while the chlorophyll fluorescence values were 54.3,572.9,974.4 μg/L and 976.4 μg/L for P.tricornutum,respectively,indicating that nitrogen enhanced the growth and proliferation of marine microalgae within a range of concentrations.Moreover,there was siginicant difference in growth response to nitrogen concentrations between S.costatum and P.tricornutum.In general,S.costatum showed stronger ability in adapting to low nitrogen concentration than P.tricornutum did.Therefore,it can be assumed that the nitrogen concentration be of particular importance in the growth and proliferation of marine microalgae,and that the difference in the adaptation to nitrogen among microalgae be a key factor in determination of the dominance of mircoalgae in the aquatic environments.

关 键 词：中肋骨条藻 三角褐指藻 叶绿素荧光 氮 赤潮 

分 类 号：S963.213[农业科学—水产养殖]