利用间歇浸没式生物反应器进行香蕉(Musa AAA Cavendish var.Williams B6)组培快繁研究(英文)  

Micro-propagation of banana (Musa AAA Cavendish var. Williams B6) in temporary immersion bioreactor system (TIBS)

作　　者：杨柳[1,2] 秦钢 粟靖[1] 杨丽涛[1] 罗瑞鸿 李小泉 林贵美 邹瑜 李杨瑞[1,2] 

机构地区：[1]广西大学农学院,南宁530005 [2]广西作物遗传改良生物技术重点实验室,南宁530007 [3]广西美泉新农业科技有限公司,南宁530007

出　　处：《果树学报》2010年第6期1010-1013,共4页Journal of Fruit Science

基　　金：国家科技支撑计划(2007BAD30B03);广西省自然科学基金(0832087)

摘　　要：以香蕉(MusaAAACavendishvar.WilliamsB6)茎尖诱导的组培苗为材料,开展了利用间歇浸没式生物反应器(TIBS)进行组培快繁技术体系的研究。结果表明,(1)TIBS系统可使香蕉组织培养一代增殖14倍以上,比传统方法提高2～3倍;(2)含2.0mg·L-16-BA的TIBS系统适合于增殖培养;(3)TIBS系统中,以第7代继代材料为宜,接种密度在每株100mL培养基最有利于香蕉组培苗的增殖和生长;(4)TIBS系统中浸没间歇频率在4min/3h时增殖和生长表现比较优异,而4min/6h时则有利于根的诱导和根的生长。A new protocol using temporary immersion bioreactor system （TIBS） was established for micro-propagation of banana （Musa AAA Cavendish var. Williams B6）. Compared with semi-solid method, a higher multiplication rate and better morphological characteristics were obtained in TIBS. Adding 2.0 mg·L^-1 6-BA to TIBS was good for banana proliferation and growth. The materials from the 7th subcuhures resuhed in a higher muhiplication rate than other subcuhures. 100 mL of medium per inoculum produced a multiplication rate of over 13 times. The immersion frequency of 4 min/3 h was good for multiplication and 4 min/6h suitable for rooting.

关 键 词：香蕉 组培快繁 间歇浸没式生物反应器(TIBS) 

分 类 号：S668.1[农业科学—果树学]