鄱阳湖中华鳖染色体核型、G带和Ag-NORs的分析  

Analysis on Karyotype,G-Band and Ag-NORs of Chromosome of Turtle,Trionyx s.Sinensis from Poyang Lake

作　　者：王军花[1] 饶友生[1,2] 龚贵如[1] 洪一江[1] 

机构地区：[1]南昌大学生物科学系,江西南昌330031 [2]江西教育学院生物技术研究所,江西南昌330029

出　　处：《南昌大学学报（理科版）》2010年第5期495-497,共3页Journal of Nanchang University(Natural Science)

基　　金：江西省科技支撑基金资助项目(2010)

摘　　要：以常规方法制备鄱阳湖中华鳖的染色体标本,分析其核型。其核型公式为2n=66=4M+8Sm+16T+38mc,NF=78。用胰酶法制备染色体G-带,绘制了G带模式图。采用了稍加修改的Foresti方法研究鄱阳湖中华鳖染色体的Ag-NORs核型,其Ag-NORs数目主要为2个,多态性主要表现为银染粒的大小和染色的深浅,数目的多态不显著。比较鄱阳湖中华鳖和其它一些已研究过的龟鳖类动物的银染核型,绝大多数种类的Ag-NORs数为2,这说明龟鳖类动物rDNA非常保守,该类动物的演化过程较为缓慢。The chromosome preparation of turtle,Trionyx s.sinensis from Poyang Lake was made by the normal air-dried method.The karyotype of the turtle is 2n=66=4M＋8Sm＋16T＋38mc,NF=78.The G-banding of chromosomes were made by the trypsinase method,and the schema chart of it was mapped out.The Ag-NORs was studied with the improved Foresti method.The number of Ag-NORs is 2 in its chromosome.Results indicate that the rDNA is very conservative,and the evolution process of this animal was slowness.

关 键 词：中华鳖 核型 G-带 AG-NORS 鄱阳湖 

分 类 号：Q958.8[生物学—动物学]