NaCl胁迫对葡萄愈伤组织中白藜芦醇含量及细胞膜保护酶活性的影响  

Effect of NaCl Stress on resveratrol content and protection enzyme activities in callus of Vitis vinifera

作　　者：方艳[1] 李胜[1,2] 马绍英[1,2] 薛冲[1] 罗丽媛[1] 刘浩[1] 张真[1] 刘媛[1] 

机构地区：[1]甘肃农业大学生命科技学院,甘肃兰州730070 [2]兰州汇通生物科技有限公司,甘肃兰州730000

出　　处：《甘肃农业大学学报》2010年第5期67-71,78,共6页Journal of Gansu Agricultural University

基　　金：国家星火项目(2008GA860007);甘肃省科技支撑计划(0804NKCA081)

摘　　要：以‘黑比诺’葡萄试管苗叶片诱导并继代1a的愈伤组织作为试验材料,接种后分别添加0、0.1、0.2、0.3、0.4mol·L-1的NaCl溶液,研究不同浓度NaCl对愈伤组织中白藜芦醇含量及细胞膜保护酶活性的影响.结果表明:随着NaCl浓度的升高,葡萄愈伤组织中白藜芦醇的含量逐渐升高,当NaCl浓度为0.3mol·L-1时白藜芦醇含量最高,达到80.76μg·g-1,此时SOD活性也达到最高,为53.7320U·g-1·min-1;较低浓度的NaCl有助于PPO与CAT活性的升高.偏相关分析结果表明,不同浓度NaCl处理下葡萄愈伤组织中白藜芦醇的含量均与CAT活性呈显著正相关,与SOD、PPO活性呈显著负相关;不同程度NaCl胁迫下,细胞膜保护酶间的相关性也出现相应变化.The callus,which was induced from the leaves of Vitis vinifera（cv.Pinot Noir）and subcultured for 1year,was used as material to study the effect of NaCl at different concentrations（0,0.1,0.2,0.3,0.4mol·L-1） on resveratrol（Res） content and protection enzyme activities.The results showed that Res content enhanced gradually as the salt concentration increased,the maximum content of 80.76 μg·g-1 was found when NaCl concentration at 0.3mol·L^-1;meanwhile SOD reached the highest activity of 53.7320U·g^-1·min^-1.A lower concentration of NaCl（0.1,0.2mol·L^-1） increased PPO and CAT activities.Partial correlation analysis showed that Res contents were significantly positive correlated with CAT activity;however,there was a negative correlation with SOD and PPO activities.The correlation among protective enzyme activities varied with the degree of salinity.

关 键 词：葡萄 愈伤组织 NACL胁迫 白藜芦醇 细胞膜保护酶 

分 类 号：S663.1[农业科学—果树学]