四种手性农药在CHIRALPAK AS-H柱上的直接拆分  

Chiral resolution of four racemic pesticides on CHIRALPAK AS-H chiral column by high performance liquid chromatography

作　　者：聂铭[1] 赵国虎[2] 赵军[3] 韩小茜[4] 

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]兰州城市学院化学与环境科学学院,甘肃兰州730070 [3]甘肃农业大学理学院,甘肃兰州730070 [4]兰州交通大学化学与生物工程学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2010年第5期143-146,151,共5页Journal of Gansu Agricultural University

基　　金：甘肃省教育厅高等学校研究生导师科研项目(0811-07);甘肃省科技厅自然科学基金项目(096RJZA064)

摘　　要：采用高效液相色谱手性固定相在CHIRALPAK AS-H柱上对三唑醇、戊唑醇、烯唑醇和顺式高效氯氟氰菊酯4种手性农药进行了直接拆分,同时考察了流动相中极性改性剂醇的种类及含量对手性拆分的影响.结果表明:在室温及0.5mL·min-1流速下,4个手性农药样品均获得基线分离,其中三唑醇的最佳拆分条件为V正己烷:V乙醇=95:5,Rs=2.00;戊唑醇、烯唑醇和顺式高效氯氟氰菊酯的最佳拆分条件均为V正己烷:V异丙醇=90:10,Rs分别为1.40、2.76和1.80.Chiral resolution of four kinds of racemic pesticides（triadimenol,tebuconazole,diniconazole andα-lambda-cyhalothrin） was performed by high performance liquid chromatography making use of CHIRALPAK AS-H chiral column,respectively.The effect of the alcohol type,the polar modifier,and content in the mobile phase for chiral resolution was investigated.The results showed that the baseline separations were obtained under the optimal resolution conditions and the flow-rate 0.5ml·min-1 as well as the room temperature,the optimal resolution conditions for triadimenol is Vn-hexane：Vethanol=95：5as the mobile phase,Rs=2.00,and for tebuconazole,diniconazole andα-lambda-cyhalothrin are Vn-hexane：Visopropanol =90：10as the mobile phase,Rs=1.40,2.76,1.80,respectively.

关 键 词：农药 高效液相色谱 手性拆分 

分 类 号：S482[农业科学—农药学]