The Influence of Magnetite Nanoparticles on Human Leukocyte Activity  

作　　者：Anezka Dzarova Martina Dubnickova Vlasta Zavisovva Martina Koneracka Peter Kopcansky Hubert Gojzewski Milan Timko 

机构地区：[1]Institute of Experimental Physics, Slovak Academy of Sciences, Kosice 040 01, Slovakia [2]Departments of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Bratislava 832 32, Slovakia [3]Institute of Physics, Poznan University of Technology, Poznan 60-965, Poland

出　　处：《Journal of Life Sciences》2010年第5期37-43,共7页生命科学（英文版）

摘　　要：In this contribution the influence of chemically synthesized magnetite particles coated by sodium oleate and PEG （MPEG）, and magnetosomes （MS） was gradually tested on the process of phagocytosis and the metabolic activity （lysozyme and peroxidase activity） in leukocyte. Lysozyme activity is oxygen-independent liquidation mechanisms of engulfed microorganism, peroxidase activity is oxygen-dependent one. The both tested samples MS and MPEG lysed leukocyte cells during incubation. MPEG with concentration 10 and 20 μg/mL lysed almost all leukocytes and their cell viability was in the 14 ± 0.05% range. On the other hand, MS begin to influence leukocytes activity at the concentration of 1 μg/mL and this influence grows with increasing concentration up to 20 μg/mL. MS are more suitable for biological applications than MPEG which are more aggressive material than MS and their using is unavailable for these types of the test mainly for the concentration 10 - 20 μg/mL.

关 键 词：Human leukocytes activity MAGNETOSOMES magnetite particles sodium oleate PEG 

分 类 号：Q554.6[生物学—生物化学] TQ920.1[轻工技术与工程—发酵工程]