系统性红斑狼疮抗SmD1抗体与SmD1抗原mRNA水平的关系  

Relationship between level of anti-SmD1 antibody and the mRNA level of SmD1 antigen in patients with systemic lupus erythematosus

作　　者：王奔放[1] 陈兴国 曾庆娣 王自正 

机构地区：[1]东南大学附属江阴市人民医院检验科,江苏江阴214400 [2]江苏省南京临床核医学中心,210006

出　　处：《蚌埠医学院学报》2010年第11期1141-1143,共3页Journal of Bengbu Medical College

摘　　要：目的:分析SmD1抗原的mRNA水平与抗SmD1抗体的关系。方法:收集30例抗SmD1抗体升高的系统性红斑狼疮(SLE)患者、30例抗SmD1抗体正常的SLE患者、30名健康体检者的血标本。用荧光定量RT-PCR检测SLE组和正常对照组SmD1抗原的mRNA水平。所得数据应用SPSS11.5软件包进行统计分析。结果:抗SmD1抗体升高、抗SmD1抗体正常的SLE患者以及正常对照组中,SmD1抗原的荧光定量PCR结果-△△CT分别为-0.60±2.99、-0.04±1.92和-0.00±2.80,差异无统计学意义(P>0.05)。结论:SmD1基因的表达在各组中无明显不同,SLE患者免疫异常,表型特别丰富,几乎涉及整个免疫系统,仅以SmD1mRNA表达异常不可能完全解释免疫系统的紊乱。Objective：To analyze the relationship between the mRNA level of SmD1 antigen and the level of anti-SmD1 antibod in patients with systemic lupus erythematosws（SLE）.Methods：The blood samples from 30 SLE patients with high level of anti-SmD1 antibody,30 cases with normal level of anti-SmD1 antibody and 30 normal controls were collected.The mRNA level of SmD1 antigen was detected by real-time PCR in all the cases.SPSS11.5 was used for statistical analysis.Results：In the SLE patients with high level of anti-SmD1 antibody,patients with normal level of anti-SmD1 antibody and the normal controls,-△△CT of real-time PCR was-0.60±2.99,-0.04±1.92 and-0.00±2.80,respectively.The difference was not significant（P0.05）.Conclusions：The gene expression level of SmD1 has no significant different among the three groups.The immune abnormity in the cases with SLE is almost involved in the whole immune system.It is impossible to explain the disorder of immune system by the abnormity of mRNA level of SmD1 antigen.

关 键 词：红斑狼疮 全身性 抗SMD1抗体 荧光定量PCR 

分 类 号：R593.241[医药卫生—内科学]