转vip3基因作物研究进展  

Research Progress of vip3 Transgenic Crops

作　　者：李朋波[1] 曹美莲[1] 杨六六[1] 刘惠民[2] 李燕娥[1] 

机构地区：[1]山西省农业科学院棉花研究所,山西运城044000 [2]山西省农业科学院,山西太原030006

出　　处：《山西农业科学》2010年第11期81-84,共4页Journal of Shanxi Agricultural Sciences

基　　金：转基因生物新品种培育重大专项(2008ZX08010-3;2009ZX08005-007B);山西省自然基金项目(2009011038-2);山西省农业科学院博士研究基金项目(YBSJJ0905)

摘　　要：苏云金芽孢杆菌(Bacillus thuringiensis,Bt)杀虫晶体蛋白(Insecticidal Crystal Proteins,ICPs)转基因作物在全球种植已近15年。随着转基因作物种植面积的扩大,害虫对Bt杀虫蛋白产生抗性已成为一个严峻的问题。通过研究发现,苏云金芽孢杆菌的营养生长阶段可产生一种新的毒素,即VIP3蛋白。VIP3蛋白与已知的ICPs没有序列同源性和结构相似性,表达VIP3对十多种鳞翅目害虫具有毒杀作用,具有更广泛的抗虫谱,尤其可毒杀小地老虎(Agrois ipsilon)等对Bt非敏感害虫,对环境生物没有不良影响,是防治抗性害虫及扩大抗虫谱的一种新策略。ICPs gene was the most successful target gene and its transgenic crops have been cuhivated for about 15 years over the world. With the increasing use of Bt transgenic crops, insect resistance to Bt protein are likely to become a serious problem. Some research showed that at vegetative growth stage, B thuringiensis could produce a new toxin, i. e. vegetative insecticidal proteins （ VIP ）. There are no sequence homology and structural similarity between VIP3 and ICPs toxic protein. It had more than 10 kinds of toxic to lepidopteron pests, VIP3 protein of vip3 transgenic has a broader spectrum of insect-resistant, particular to the black cutworm and other pests on Bt non-sensitive. There are no adverse effects on environmental organism, rip3 transgenic crop become a new strategy to control ICPs resistant pests and broaden insecticidal spectrum.

关 键 词：vip3 ICPs抗性害虫 抗虫谱 转基因作物 

分 类 号：S188[农业科学—农业基础科学]