一株抗多杀性巴氏杆菌的白花泡桐内生真菌的分离和鉴定  

Isolation and Identification of an Endogenous Fungus Strain with Anti-Pasteurella multocida from Paulownia fortunei

作　　者：罗江华[1] 吾鲁木汗.那孜尔别克 李科[1] 刘祝祥[1] 龚凤娟[1] 恩特马克.布拉提白 

机构地区：[1]吉首大学生物资源与环境科学学院省部级共建生物工程实验室,湖南吉首416000

出　　处：《贵州农业科学》2010年第11期165-168,共4页Guizhou Agricultural Sciences

基　　金：吉首大学研究生科研创新项目"泡桐抗菌活性内生真菌的筛选及其代谢产物的初步研究"(JGY200910);湖南省研究生科研创新项目"湘西地区泡桐属植物分子分类学研究"(CX2009B198)

摘　　要：采用组织分离法从药用植物白花泡桐叶中分离内生真菌,以动物病源菌多杀性巴氏杆菌C48-3、C51-3和X-73为指示菌种对分离到的7株内生真菌进行了抗菌活性筛选,采用形态特性观察及ITS-rRNA序列分析对具有抗菌活性菌株JSD-8进行鉴定。抑菌试验结果显示,JSD-8菌株对多杀性巴氏杆菌C48-3、C51-3和X-73均具有较强的抗菌活性,其抑菌活性成分值得进一步研究。通过形态特性观察及ITS-rRNA序列分析,将JSD-8鉴定为串珠状赤霉菌。The anti-Pasteurella multocida activity of 7 endogenous fungus strains isolated from Paulownia fortunei was screened by taking Pasteurella multocida C48-3,C51-3 and X-73 as indicating strains and JSD-8 endogenous fungus strain with anti-Pasteurella multocida activity was analyzed by morphological observation and ITS-rRNA sequence.The results from the bacteriostasis test showed that JSD-8 endogenous fungus strain had strong activity against Pasteurella multocida and its bacteriostasis activity composition should be further studied.JSD-8 endogenous fungus strain with anti-Pasteurella multocida activity is identified as Gibberella moniliformis by morphological observation and ITS-rRNA sequence analysis.

关 键 词：多杀性巴氏杆菌 内生真菌 鉴定 ITS-rRNA 

分 类 号：S858.315.1[农业科学—临床兽医学]