三种肌松药对人离体静脉血中性粒细胞CD11b表达的影响  

Effects of three muscle relaxants on the CD11b expression of human neutrophil in venous blood in vitro

作　　者：刘尊远[1] 张岩[2] 薛峰[3] 

机构地区：[1]潍坊医学院麻醉系,山东潍坊261041 [2]山东省潍坊市人民医院麻醉科,山东潍坊261041 [3]山东省潍坊市人民医院检验科,山东潍坊261041

出　　处：《中国医药导报》2010年第32期25-26,28,共3页China Medical Herald

摘　　要：目的:研究三种肌松药(顺阿曲库铵、哌库溴铵、维库溴铵)对人离体静脉血中性粒细胞CD11b表达的影响。方法:采集10例健康成人外周静脉血,每一份血样均用于下列各组研究,实验分为14组,空白组;静息无刺激下药物各组[顺阿曲库铵1,2浓度组(0.5/0.1μg/ml);哌库溴铵1,2浓度组(0.5/0.1μg/ml);维库溴铵1,2浓度组(0.5/0.1μg/ml)];脂多糖(LPS)组;刺激下药物各组[三种1,2浓度(0.5/0.1μg/ml)肌松药分别添加脂多糖组]。各处理组血样预先加入相应浓度药物,空白组和LPS组加入等容量生理盐水。37℃避光孵育1.5 h;LPS组和刺激下各组再加入终浓度为1μg/ml的脂多糖进行刺激,空白组和静息下各组分别加入等容积生理盐水;37℃避光孵育2 h。而后采用流式细胞仪测定各组CD11b表达。结果:与空白对照组比较,低浓度维库溴铵和哌库溴铵均可抑制中性粒细胞表面CD11b表达(P<0.05);两浓度顺阿曲库铵对中性粒细胞表面CD11b表达均无影响;三种肌松药对LPS强刺激下的中性粒细胞表面CD11b表达均无影响。结论:接近临床肌松维持浓度的维库溴铵和哌库溴铵对静息状态人离体静脉血中性粒细胞表面CD11b表达有抑制作用。Objective： To study the effects of three muscle relaxants on the CD11b expression of polymorphonuclear neutrophil（PMN） of healthy human in vitro.Methods： Peripheral venous whole blood samples were collected from 10 middle-aged healthy donors.Each blood sample was divided into 14 groups： blank group;static groups,which included six groups only added with different concentrations of muscle relaxants [Cisatracurium（0.5/0.1 μg/ml）,Pipecuronium（0.5/0.1 μg/ml）,vecuronium（0.5/0.1 μg/ml）];LPS group;stimulated groups,which included six groups added with different concentrations of muscle relaxants and LPS [three muscle relaxants with two different concentrations above（0.5/0.1 μg/ml） added with LPS].12 treated groups with different concentrations of muscle relaxants were incubated for 1.5 h at 37 ℃ in the dark,while the blank group and LPS with normal saline（NS） instead.Then,LPS group and stimulated groups were added LPS,the concentration of LPS reached 1 μg/ml,other groups were incubated for 2 h at 37℃ in the dark.Finally,CD11b expression of all groups was determined using flow cytometry.Results： Compared with the blank group,low concentration of Pipecuronium and Vecuronium groups all could suppress the CD11b expression of PMN in static state.Two concentration of Cisatracurium had no effect on the CD11b expression of PMN in static state.Three muscle relaxants had no effect on the CD11b expression of LPS-activated venous blood in vitro.Conclusion： The concentration of Vecuronium and Pipecuronium that approximates clinical maintenance dose has effect of inhibiting the CD11b expression of LPS-activated venous blood in vitro.

关 键 词：肌松药 顺阿曲库铵 哌库溴铵 维库溴铵 中性粒细胞 CD11B 

分 类 号：R96[医药卫生—药理学]