纳米化仿生诱导骨的构建及促进兔横突间融合研究  

An experimental study on rabbit＇ s intertransverse process fusion promoted by mimetically osteoin ductive nanoscale bone

作　　者：郭洪刚[1] 刘静[3] 姚芳莲[4] 马信龙[1] 李峰坦[2] 李晓眠[5] 

机构地区：[1]天津医科大学总医院骨科,300052 [2]天津医科大学总医院放射科,300052 [3]天津市中心妇产科医院 [4]天津大学材料学院 [5]天津医科大学

出　　处：《中华实验外科杂志》2010年第11期1627-1629,共3页Chinese Journal of Experimental Surgery

基　　金：天津市卫生局科技基金资助项目（06KZ46）

摘　　要：目的 探讨纳米化仿生诱导骨促进兔脊柱融合的可行性.方法 成骨化兔脂肪基质细胞（ADSCs）接种在纳米级β磷酸三钙/壳聚糖/聚已内酯基质,构建纳米化仿生诱导骨 建立兔腰5～6横突间融合模型,A组纳米化仿生诱导骨,B组自体髂骨,C组脂肪基质细胞移植,D组空白支架,E组rhBMP-2注射.行影像学、组织学、免疫组化、新骨及诱导因子定量分析等检查.结果 A组融合能力最强,融合率100%、新骨面积比（87.32±1.68）%、BMP含量及力学指标（128±19）、（331.56±3.21）N、（526.78±3.19）Nmm均高于其他组,差异有统计学意义（P＜0.05） B、C组次之,E组新骨生长不明显,融合相对延缓.D组未融合.结论 仿生诱导骨能强化骨再生,促进脊柱融合.Objective To investigate the applied value of mimetically osteoinductive bone in promoting spinal fusion of rabbits. Methods With aid of biological gels compound and dynamic （oscillating pattern） seeding, three-dimensional construction of mimetically osteoinductive bone was made by implanting adipose-derived stromal cells （ADSCs） into rhBMP-2-mediated nβ-TCP/Cs/PCL matrix. Lumbar 5-6intertranverse process fusion was established in New Zealand rabbits. The rabbits in group A were transplanted by mimetically osteoinductive bone, those in group B by bone autograft, those in groups C and E implanted by ADSCs and rhBMP-2 respectively, and those in group D given nβ-TCP/Cs/PCL matrix as blank scaffold. Histology, immunohistochemistry, new bone ratio, BMP quantification, and palpation and biomechanical examinations were performed at different periods. Results Compared with other groups,group A displayed remarkable fusion capacity with statistically significant difference according to data of new bone ratio and BMP content and biomechanical testing [100% of fusion rate, （ 87. 32 ± 1.68 ） % of new bone ratio, （ 128 ± 19） of BMP content, （331.56 ±3.21 ） N, （526. 78 ±3. 19） Nmm,P 〈0. 05].Conclusion Enhanced osteogenic potential can be achieved by using mimetically osteoinductive bone and spinal fusion can be promoted with concord pattern of osteoinductive and osteoconductive effects.

关 键 词：脊柱融合 仿生诱导骨 脂肪基质细胞 

分 类 号：R687.3[医药卫生—骨科学]