携带缓释药物的生物可降解输尿管支架体内外降解特性研究  

Biodegradation characteristics of a new kind of drug-eluting biodegradable ureteral stent in vivo and in vitro

作　　者：符伟军[1] 曹磊[1] 徐福强[1] 李钢[1] 王忠新[1] 洪宝发[1] 王晓雄[1] 胡堃[2] 崔福斋[2] 张旭[1] 

机构地区：[1]解放军总医院泌尿外科,北京100853 [2]清华大学材料科学与工程系

出　　处：《中华实验外科杂志》2010年第11期1716-1718,共3页Chinese Journal of Experimental Surgery

基　　金：北京市自然科学基金资助项目（2092029）;全军十一五计划项目面上课题资助项目（06MA298）;解放军总医院科技创新苗圃基金资助项目（08MP08）

摘　　要：目的 观察携带缓释药物的生物可降解输尿管支架的体内外降解特性,比较输尿管裸支架与输尿管药物支架的生物相容性等差异.方法 将输尿管裸支架与输尿管药物支架分别浸泡于新鲜人体尿液中,观察体外支架降解.将两种输尿管支架分别包埋于18只Wistar大鼠脊柱两侧肌肉内.通过组织切片染色、扫描电镜、相对分子质量测定及质量变化分析体内生物可降解特性差异.结果 输尿管药物支架体外人体尿液浸泡10周时,支架失重率已达35%.切片染色发现组织反应较轻,纤维增生不明显.扫描电镜支架表面失去原有形态,出现大块状脱落物.12周时,降解为细小泥沙样颗粒输尿管裸支架组纤维膜厚（21.24±3.27）μm,输尿管药物支架组纤维膜厚（10.75±3.69）μm,两组比较差异有统计学意义（P＜0.01）.结论 与输尿管裸支架比较,输尿管药物支架的降解特性相似,但炎症反应轻,包裹纤维膜厚度更薄,具有良好的组织相容性.Objective To observe the degradation characteristics of the novel drug-eluting biodegradable polylactide ureteral stents in vivo and in vitro, and to compare biocompatibility differences of the pure biodegradable ureteral stents with paclitaxel-eluting biodegradable ureteral stent. Methods The polylactide ureteral stents were dipped into fresh urine specimen and urinary environment simulated in vivo by shocks. And in vivo these two kinds of ureteral stents were implanted in the dorsal muscle of 16 Wistar rats. Histological study was used to investigate the tissue reaction of the material and the differeces of these two kind of ureteral stents were evaluated. Scanning election microscopy （SEM） and measurement of gravity and molecular weight were performed to make out the biodegradation characteristics of stents in vitro and in vivo. Results After being soaked in human urine for 10 weeks, weight of the novel drug-eluting stents was decreased by 35%. Under the SEM, the surface of ureteral stent lost its original shape, shedding a large number of blocks. Histological staining found that fibrosis of drug-eluting biodegradable ureteral stent was mild. After 12 weeks, it was degraded into small sand-like particles. The fiber membrane thickness of pure stent was （21.24 ±3. 27） μm and of drug-eluting stents was （ 10. 75 ±3.69） μm. There was significant difference between two groups （P 〈 0. 01 ）. Conclusion The biodegradation of drug-eluting stents is similar to pure biodegradable ureteral stent, but the inflammatory reaction of the former is minor and the fiber membrane is thinner. The drug-eluting stent has better histocompatibility and can be used for stenting after a ureteral repair.

关 键 词：输尿管 支架 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]