急性大鼠脊髓腹侧压迫伤的模型制备  

Preparation of acute anterior spinal cord injury models

作　　者：李剑锋[1] 闫金玉[1] 张飞[2] 张旭[1] 刘遵南[1] 

机构地区：[1]内蒙古医学院第二附属医院康复科,呼和浩特010030 [2]内蒙古自治区医院普外二科

出　　处：《中华实验外科杂志》2010年第11期1740-1742,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30860300）

摘　　要：目的 建立急性压迫型大鼠前脊髓综合征模型.方法 将Wistar大鼠放置腹侧致伤钩对其腹侧脊髓形成压迫,造成不同程度脊髓损伤模型,并进行行为学及神经电生理评价.结果 造模后各实验组斜板角度分别为A组（52.89±7.31） B组（47.08±7.27） C组（41.32±7.85） BBB评分分别为A组（8.47±4.14） B组（3.48±3.17） C组（0.83±3.06） 均较假手术组明显减小（P＜0.01）,轻、中、重度损伤组之间差异有统计学意义（P＜0.05）.手术8周后各实验组MEP的潜伏期明显高于对照组（P＜0.05）,各实验组间差异有统计学意义（P＜0.05） 重度损伤组MEP的潜伏期为（5.74±0.12）,明显高于对照组（P＜0.05）.结论 前脊髓损伤综合征模型出现明显的行为学、神经电生理及病理学改变,而且可以制备出不同程度前脊髓损伤综合征模型.Objective A new model of acute anterior spinal cord injury was made to explore the pathophysiologic mechanism of spinal cord injurys. Methods The metal hook was fixed in front of the spinal cord of Wistar rats, and the praxiology and nerve electrophysiology measurements were carried out on different kinds of anterior spinal cord injury models. Results The gradient in groups A, B, C was （52. 89±7.31）, （47.08 ±7.27） and （41.32±7.85）, and BBB score in groups A, B and C was （8.47 ±4. 14）, （3.48 ±3. 17） and （0. 83 ±3.06） respectively, which was significantly reduced as compared with those in blank control group after operation （P 〈 0. 01 ）, and there was significant difference among groups A, B, C （P 〈 0. 05 ）. Motor evoked potential （MEP） in experiment groups was significantly longer than control group at the 8th week after operation （P 〈0. 05）, and there was significant difference among experimental groups （ P 〈 0. 05 ）. MEP in severe traumatic group was significantly longer （ P 〈 0. 05 ）. Conclusion There was obvious praxiology, pathology and nerve electrophysiology changes in anterior spinal cord injury models, and furthermore, it could be used to prepare the models to varying degrees.

关 键 词：急性前脊髓综合征 压迫 模型 动物 

分 类 号：R687.3[医药卫生—骨科学]