正交试验优化养精种玉汤提取工艺的研究  

Optimization of extraction process of Yangjing Zhongyu Decoction by orthogonal test

作　　者：马红霞[1] 王岩[2] 吴慧琳[2] 王婴[2] 

机构地区：[1]广州医学院第一附属医院,广东广州510120 [2]广东药学院,广东广州510006

出　　处：《中草药》2010年第11期1800-1803,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30701119);中山市科技计划项目(2009H019);广东药学院师资队伍建设经费资助

摘　　要：目的优化养精种玉汤的提取工艺,并考察合煎液和单煎液中马钱苷、芍药苷和阿魏酸的变化。方法采用正交试验法优选工艺,并在同一色谱条件下进行3种成分的HPLC测定,其中马钱苷、芍药苷的检测波长为230nm,阿魏酸的检测波长为316 nm。结果 最佳提取条件为用6倍量水提取3次,每次1 h。结论优选工艺稳定可行,合煎液中的马钱苷、芍药苷和阿魏酸量较单煎液均有不同程度的提高。Objective To optimize the extraction process of Yangjing Zhongyu Decoction and investigate the content change of loganin,paeoniflorin,and ferulic acid in the mixed and seperated decoctions.Methods Orthogonal design was used to optimize the extraction process.The contents of the three components were determined under the same chromatographic conditions by HPLC.The detective wavelength of loganin and paeoniflorin was 230 nm,and the detective wavelength of ferulic acid was 316 nm.Results The optimum extraction condition was as follows： six times water,extracting three times,every time for an hour.Conclusion The optimum process is stable and feasible. The contents of the three components are more improved in the mixed decoction in varying degrees than single decoction.

关 键 词：养精种玉汤 提取工艺 正交试验 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]