新型双稀土配合物掺杂的Ag@SiO_2纳米粒子的制备及表征  

Preparation and Characterization of A Novel Type of Dual-Lanthanide-Complexs Doped Ag@Silica Fluorescent Nanoparticles

作　　者：尹东光[1] 张礼[1] 刘斌虎[1] 谢春娟[1] 张乐[1] 胡艳[1] 

机构地区：[1]上海大学环境与化学工程学院,上海200444

出　　处：《化学学报》2010年第19期1965-1970,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(No.40830744);上海市浦江人才计划项目(D类);上海市纳米专项项目(No.0752NM024);上海市重点学科(No.S30109);国家973项目(No.2010CB933901);教育部留学回国人员科研启动基金资助项目

摘　　要：首先合成配合前驱体对氨基苯甲酸(PABA)-二乙烯三胺五乙酸(DTPA)-3-氨丙基三甲氧基硅烷(APTMS)及双稀土配合物Eu3+/Tb3+-PABA-DTPA-APTMS,然后采用反相微乳液法成功制备出表面带氨基的核壳型稀土配合物Eu3+/Tb3+-PABA-DTPA-APTMS掺杂的Ag@SiO2荧光纳米粒子.利用透射电子显微镜、荧光光谱、紫外-可见光谱等手段进行表征,并进行了光稳定性及氨基测定等实验,结果表明,该纳米粒子中Eu3+与Tb3+在最大发射峰处的荧光强度较Eu3+/Tb3+-PABA-DTPA-APTMS掺杂的没有银核的SiO2荧光纳米粒子分别提高了3.0和3.4倍,所制备的纳米粒子呈规则球状,具有良好的分散性和光稳定性,纳米粒子表面带有氨基,可不需要进行表面修饰而直接与生物分子反应.该纳米粒子有望作为一种新型的稀土荧光探针应用于高灵敏检测的时间分辨荧光免疫分析、生物传感器、生物芯片等.A novel type of dual-lanthanide-complexs doped Ag@silica fluorescent nanoparticles with amino functionalized has been developed by a facil water-in-oil （W/O） microemulsion method. The structural and optical properties of the nanoparticles were characterized by transmission electron microscopy （TEM）, fluorescence spectra and UV-Vis spectra, respectively. The results show that the fabricated nanoparticles are spherical, core-shell structure, highly fluorescent and highly photostable. Measurements of the luminescent emissions from the Ag@SiO2 nanoparticles, in comparison with control silica nanoparticles without silver cores, showed that the presence of the silver cores can increase the fluorescence intensity approximately 3-fold. This enhancement offers a potential increase in detection sensitivity with increased photostability. The nanoparticles are expected to be utilized in super-sensitive bioassays systems such as time-resolved fluorescence immunoassay, biosensor and biochip.

关 键 词：对氨基苯甲酸-二乙烯三胺五乙酸-3-氨丙基三甲氧基硅烷 Eu3+ TB3+ Ag@SiO2纳米粒子 金属增强荧光 

分 类 号：O641.4[理学—物理化学]