黄芪甲苷抑制迟发型超敏反应的作用  

Inhibition of Astragaioside Ⅳ on Delayed Type Hypersensitivity

作　　者：洪敏[1] 郑劼[1] 匡烨[1] 王亮[1] 

机构地区：[1]南京中医药大学国家规范化中药药理实验室江苏省方剂研究重点实验室,江苏南京210046

出　　处：《南京中医药大学学报》2010年第6期442-444,I0004,I0005,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省方剂研究重点实验室开放课题;南京中医药大学基础研究重点培育项目

摘　　要：目的考察黄芪甲苷(AS-IV)对迟发型超敏反应的影响。方法应用2,4-二硝基氯苯(DNCB)造成小鼠变态反应性接触性皮炎(ACD)模型,分别考察(AS-IV)诱导相和效应相给药对耳廓肿胀的影响,并进行耳病理组织学检查;ELISA方法检测小鼠血清IFN-γ水平。MTT法检测ConA诱导的脾细胞增殖。结果 (AS-IV)诱导相给药可明显抑制ACD小鼠耳肿胀,而效应相给药未观察到明显效应;病理组织学结果显示(AS-IV)诱导相给药可减轻模型小鼠局部水肿及血管扩张,减少淋巴细胞浸润;(AS-IV)诱导相给药明显降低血清IFN-γ水平;离体实验未发现(AS-IV)对ConA诱导的脾细胞增殖有明显影响。结论 (AS-IV)诱导相给药有抑制迟发型超敏反应的作用,该作用可能与抑制IFN-γ有关。OBJECTIVE To evaluate the effects of astragaioside Ⅳ{AS-Ⅳ） on delayed type hypersensitivity （DTH）. METHOD Murine allergic contact dermatitis （ACD） models were induced by DNCB in AS-Ⅳ induction phase and reaction phase to observe their effects and conduct the auricle histopathological examination respectively. Mice IFN-γ in serum was detected by ELISA. Splenocytic proliferation induced by ConA was measured by TTA. RESULT AS-IV induction phase evidently inhibited the auricle inflammation of ACD models; however, effector phase had no obvious effect. Hestopathological results indicated that AS-Ⅳ induction phase alleviated local edema and angiectasis of mice models and reduced lymphocytic infiltration. AS-Ⅳ induction phase markedly decreased IFN-γ levels in serum. And in vitro study, AS- Ⅳ had no obvious effect on proliferation of splenocytes induced by ConA. CONCLUSION AS-Ⅳ induction phase can restrain DTH ,which may be due to its inhibition to IFN-γ.

关 键 词：黄芪甲苷 迟发型超敏反应 诱导相 IFN-Γ 

分 类 号：R285.5[医药卫生—中药学]