Transcriptional Regulation by HSV-1 Induced HTRP via Acetylation System  

作　　者：Jie CHEN Yan-mei LI Jian-feng LI Long-ding LIU Yun LIAO Rui-xiong NA Jing-jing WANG Li-chun WANG Qi-han LI 

机构地区：[1]Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming 650118, China

出　　处：《Virologica Sinica》2010年第6期417-424,共8页中国病毒学（英文版）

基　　金：The National Natural Science Foundation of China (30670094);the Ph.D. Programs Foundation of Ministry of Education of China (20060023008)

摘　　要：The protein HTRP (human transcription regulator protein) is encoded by the differential gene htrp and induced by Herpes simplex virus type 1 (HSV-1) infection in KMB-17 cells.HTRP was found to interact with SAP30 (mSin3A Association Protein),one of the components of co-repressor complex mSin3A,which is part of the deacetylation transfer enzyme HDAC.To reveal the biological significance of the interaction between HTRP and SAP30,real-time PCR and a dual-luciferase detecting system was used.The results indicate that HTRP could inhibit the transcription of a viral promoter,whose interaction with SAP30 synergistically affects transcriptional inhibition of the viral genes,and is related to HDAC enzyme activity.ChIP experiments demonstrate that HTRP could promote HDAC activity by increasing the deacetylation level of lysine 14 and lysine 9 in histone H3.The protein HTRP （human transcription regulator protein） is encoded by the differential gene htrp and induced by Herpes simplex virus type 1 （HSV-1） infection in KMB-17 cells.HTRP was found to interact with SAP30 （mSin3A Association Protein）,one of the components of co-repressor complex mSin3A,which is part of the deacetylation transfer enzyme HDAC.To reveal the biological significance of the interaction between HTRP and SAP30,real-time PCR and a dual-luciferase detecting system was used.The results indicate that HTRP could inhibit the transcription of a viral promoter,whose interaction with SAP30 synergistically affects transcriptional inhibition of the viral genes,and is related to HDAC enzyme activity.ChIP experiments demonstrate that HTRP could promote HDAC activity by increasing the deacetylation level of lysine 14 and lysine 9 in histone H3.

关 键 词：Herpes simplex virus type 1 （HSV-1） HTRP SAP30 Transcription regulation 

分 类 号：Q756[生物学—分子生物学] S722.11[农业科学—林木遗传育种]