低温对‘寒富’苹果花芽渗透调节物质含量和膜脂过氧化的影响  

Effect of Low Temperature on 'Hanfu' Apple Buds Osmoticum Content and Membrane Lipid Peroxidation

作　　者：马怀宇[1] 吕德国[1] 刘国成[1] 秦嗣军[1] 

机构地区：[1]沈阳农业大学园艺学院,北方果树育种与生理生态研究所,辽宁沈阳110161

出　　处：《北方园艺》2010年第21期1-4,共4页Northern Horticulture

基　　金：沈阳农业大学校青年基金资助项目(2006-02-03);辽宁省教育厅资助项目(01042608006);国家苹果产业技术体系苹果专项资助项目(nycytx-08-03-05)

摘　　要：以‘寒富’苹果的花芽为试材,设置梯度低温(-25、-30、-35和-40℃)处理,研究顶花芽和腋花芽的渗透调节物质含量对低温的响应特点及对花芽低温适应性的影响。结果表明:随着温度下降,顶花芽和腋花芽的游离氨基酸含量变化趋势相似,但顶花芽的游离氨基酸含量一直高于腋花芽。腋花芽的可溶性糖和可溶性蛋白含量均是在-35℃达到高峰后显著下降;顶花芽的则是在-35℃达到最低点后显著升高。腋花芽的脯氨酸含量在-35℃达到高峰后保持在较高水平,顶花芽的则是在-35℃达到最低点后显著升高。腋花芽的MDA含量在-35℃之前保持在较低水平,此后迅速上升;顶花芽的MDA含量总体上呈上升趋势,且明显高于腋花芽。由此认为,腋花芽的渗透调节物质对低温的响应能力强于顶花芽,应是腋花芽低温适应性强于顶花芽的一个重要原因。In order to research the response characteristic of osmoticum in terminal flower buds and lateral flower buds under low temperature treatment（-25,-30,-35,-40℃）,＇Hanfu＇ apple flower buds were used as materials in this experiment.The results showed that the variation of free amino acid content in two kinds of flower buds was in the similar tendency with the temperature decreasing,while free amino acid content in terminal flower buds was higher than that of lateral flower buds all along.Soluble sugar and soluble protein content in lateral flower buds reached to the highest point at-35℃ and then dropped significantly,which reached to the lowest points at-35℃ and then rose obviously in the terminal flower buds.Proline content of lateral flower buds reached to the highest point at-35℃ and kept at a relatively higher level after-35℃,which reached to the lowest point at-35℃ and then increased significantly in terminal flower buds.MDA content of lateral flower buds kept in a lower level before-35℃,which presented an upward tendency in apical flower buds as a whole and was apparently higher than that of lateral flower buds.It could be seen that the respond capability of osmoticum in lateral flower buds was stronger than that of in terminal flower buds,which would be one of important reasons for higher low temperature suitability of lateral flower buds.

关 键 词：'寒富’苹果 花芽 抗寒性 渗透调节物质 丙二醛 

分 类 号：S661.1[农业科学—果树学]