ω-3脂肪酸去饱和酶基因对番茄抗旱性的影响  

Effect of LeFAD7 Gene on Drought Resistance of Tomato Plants

作　　者：黄霄[1] 

机构地区：[1]临沂师范学院,山东临沂276005

出　　处：《北方园艺》2010年第21期145-149,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目（30471053）

摘　　要：为探讨番茄类囊体膜脂ω-3脂肪酸去饱和酶基因（LeFAD7基因）在干旱胁迫下对番茄抗旱性的影响,以野生型（WT）和转正义LeFAD7基因株系T（＋）-12为试材,测定了番茄叶片类囊体膜脂脂肪酸组成,干旱胁迫后的光合参数、叶绿素荧光参数和活性氧清除酶（SOD、CAT和POD）活性的含量变化。结果表明：转正义基因番茄植株类囊体膜脂中亚麻酸（18∶3）含量升高,亚油酸（18∶2）含量下降,导致膜脂脂肪酸不饱和度升高。与WT相比,干旱胁迫下转正义基因番茄植株的PSⅡ最大光化学效率（Fv/Fm）和光合速率（Pn）下降程度较小,转正义基因番茄植株能维持较高的抗氧化酶活性。干旱胁迫下LeFAD7基因对番茄起一定的保护作用。In order to investigate effact of LeFAD7 gene of thylakoid membrane on droug resistanc of tomato plants,WT and sense-transgenic lines T（＋）-12 of tomato were used to measure the fatty acid composition of thylakoid membrane,photosynthetic and Antioxidant enzyme activIty under drought stress.The results showed that linolenic acid（18∶3） obviously increased and the content of oleic acid（18∶2）concomitantly reduced in the sense-transgenic lines,resulting in the increase of the unsaturation of thylakoid memberrane lipids.The maximal photochemical efficiency of PS Ⅱ（Fv/Fm） and photosynthetic rates（Pn） in T（＋）-12 lines with higher 18∶3 decreased less under drought stress.under drought stress.Compared to WT,the sense-transgenic lines could maintain higher activities of the vigour of antioxidant enzyme.Those results indicated that LeFAD7 gene of thylakoid membrane lipids protect tomato plants on drought stress.

关 键 词：LeFAD7基因 类囊体膜脂组成 荧光参数 光合速率 抗氧化酶 干旱胁迫 

分 类 号：S641.2[农业科学—蔬菜学]