青花菜离体再生体系研究  

Study on the Regeneration System of Broccoli

作　　者：符庆功[1] 吴慧敏[1] 胡颖[1] 

机构地区：[1]浙江农林大学农业与食品科学学院,浙江临安311300

出　　处：《北方园艺》2010年第21期150-152,共3页Northern Horticulture

基　　金：浙江省教育厅优秀青年教师资助项目(2273000001)

摘　　要：以＂碧冠＂、＂谊和80＂和＂幸运＂3个青花菜品种带柄子叶与下胚轴为材料,探讨了激素组合、苗龄及AgNO3浓度等因素对外植体不定芽分化再生的影响。结果表明：在MS＋NAA 0.1mg/L＋6-BA 2.0/L＋AgNO34.0~6.0 mg/L培养基上,＂谊禾80＂4 d龄无菌苗的下胚轴和6 d龄带柄子叶可以获得较高的分化率（92.5%和85%）;3个品种再生芽在MS＋IBA 0.2 mg/L生根培养基上生根率达95%以上。Petiolate cotyledons and hypocotyls of the broccoli（Brassica oleracea var.Italica Planch.） were used as explants in the optimum plant regeneration system.The results showed that the hypocotyls frequency was 92.5% in ＂YiHe-80＂,when the explants were precultured for 4 days and placed on the MS medium supplemented with NAA 0.1 mg/L,6-BA 2.0 mg/L,AgNO3 4.0～6.0 mg/L,and the frequency of petiolate cotyledons from 6 day preculture explants was 85% in the same condition.While the root ratio of every variety was beyond 95% on the MS medium with IBA 0.2 mg/L.

关 键 词：青花菜 再生体系 下胚轴 带柄子叶 

分 类 号：Q949.784.3[生物学—植物学]