七个甜柿品种启动培养  

Frimary Culture of Seven Persimmon Cultivars

作　　者：扈惠灵[1] 周瑞金[1] 苗卫东[1] 张国付[1] 张晓丽 

机构地区：[1]河南科技学院园林学院,河南新乡453003 [2]河南汝州市第四高级中学,河南平顶山467000

出　　处：《北方园艺》2010年第21期153-155,共3页Northern Horticulture

基　　金：河南省高校科技创新人才支持计划资助项目(2008HASTIT013);河南科技学院博士科研启动基金资助项目(20070025)

摘　　要：以柿休眠芽为外植体,以(1/2N)MS+6-BA 4.0 mg/L+IAA 0.2 mg/L+PVP 0.6g/L+蔗糖3 0 g/L+6.0 g/L琼脂为培养基,对‘次郎’、‘西村早生’、‘禅寺丸’、‘兴津20’、‘新秋’、‘前川次郎’、‘阳丰’7个甜柿品种进行了初代培养研究。结果表明:‘西村早生’、‘兴津20’、‘新秋’、‘阳丰’易成活,成活率为100%,其次为‘前川次郎’和‘次郎’,成活率分别为78%和70%,‘禅寺丸’不易成活,成活率仅为9.5%;从增殖倍数来看,该培养基有利于‘禅寺丸’的增殖,增殖效果较明显,‘西村早生’次之,‘阳丰’增殖效果较差。同时研究了GA3在甜柿初代培养中的效应,表明在初代培养中,添加适量GA3更有利于甜柿休眠芽初期的启动发育和伸长生长。Primary culture was conducted on the same medium（1/2N）MS supplemented with 6-BA 4.0 mg/L,IAA 0.2 mg/L,PVP 0.6 g/L,sucrose 30 g/L and agar 6.0 g/L,and dormant buds were taken as explants from seven persimmon cultivars including ＇Jiro＇,＇Nishimurawase＇,＇Zenjimaru＇,＇Okitsu20＇,＇Xinqiu＇,＇Maekawa＇and ＇Youhou＇.The results showed that the survival rate of ＇Nishimurawase＇,＇Okitsu20＇,＇Xinqiu＇and ＇Youhou＇were high up to 100%,the second was ＇Maekawa＇（78%） and ＇Jiro＇（70%）,the lowest was ＇Zenjimaru＇（9.5%）.In the aspect of proliferation multiple,the medium was benefited proliferation of ＇Zenjimaru＇,and the effect of proliferation was obvious.The second was ＇Nishimurawase＇,the effect of proliferation of ＇Youhou＇was the worst.In addition the effect of GA3 in preliminary culture was also studied.The results indicated that the medium with GA3 was benefit beginning development and elongation growth of persimmon dormant buds.

关 键 词：柿 休眠芽 初代培养 

分 类 号：S665.2[农业科学—果树学]