金叶榆组培快速繁殖技术研究  

Study on the Technology of Tissue Culture and Rapid Propagation of Ulmus pumila cv.Jinye

作　　者：唐晓杰[1] 孟范例[1] 程广有[1] 

机构地区：[1]北华大学林学院,吉林吉林132013

出　　处：《北方园艺》2010年第21期156-158,共3页Northern Horticulture

摘　　要：在MS培养基中分别加入不同质量浓度6-苄基氨基腺嘌呤(6-BA)和吲哚丁酸(IBA)进行金叶榆组织培养,探讨快速繁殖技术。结果表明:随着培养基中激素含量增高,形成愈伤组织的量也增多;在MS+6-BA 1.0 mg/L+IBA 0.1 mg/L培养基中的不定芽增殖数量最多(18.8),无根苗在1/2MS+IBA 1.0 mg/L培养基上生根率最高(96%);金叶榆试管苗移栽到腐殖土中成活率最高(97%),河沙中成活率次之(76%),旱田土上成活率最低(58%)。The technology of tissue culture and rapidly propagation of Ulmus pumila cv.Jinye was studied in MS medium plus 6-benzyladenine（6-BA） and indolebtyric acid（IBA）.The results showed that the more hormone content in medium,the more callus will be.The number of adventitious buds in MS＋6-BA 0.5 mg/L＋IBA 0.1 mg/L were more than other medium.The good medium to induce root was 1/2MS＋IBA 1.5 mg/L（96% rooted ratio）.There was higher proportion of viability planted test-tube seedlings in the humus soil.

关 键 词：金叶榆 组织培养 繁殖 

分 类 号：S792.19[农业科学—林木遗传育种]