高效液相色谱串联三重四极杆质谱检测饲料中10种β-受体激动剂  

Determination of ten β-agonists in feeds by high performance liquid chromatography-tandem mass spectrometry

作　　者：潘云山 聂建荣[1] 朱铭立[1] 周松英[1] 胡翠萍[1] 

机构地区：[1]广州市农业标准与监测中心,广东广州510315

出　　处：《广东农业科学》2010年第11期252-254,270,共4页Guangdong Agricultural Sciences

摘　　要：建立了以高效液相色谱串联三重四极杆质谱检测饲料中福莫特罗等10种β-受体激动剂的分析方法。饲料样品中的β-受体激动剂经高氯酸溶液提取、HLB柱净化浓缩后,导入Agilent1100液相色谱串联API4000三重四极杆质谱,采用C18色谱柱分离,通过电喷雾(ESI)离子源电离,多反应监测(MRM)对10种β-受体激动剂进行定性定量分析,结果表明,10种β-受体激动剂在0.5～40.0μg/L范围内的峰面积与质量浓度线性相关(r>0.999)。0.01、0.02、0.1、0.5 mg/kg添加水平下,回收率71.2%～103%,相对标准偏差3.7%～9.8%,方法定量限(S/N=10)为0.01 mg/kg。A high performance liquid chromatography-tandem mass spectrometry method was established for the determination of ten β-agonists in feeds.β-agonists in sample were extracted by perchloric acid solution,purified and concentrated by HLB column.A C18 column was used to separate the β-agonists.Qualitative and quantitative analysis of ten β-agonists which ionized by electrospray ionization interface（ESI） was carried out by multiple reaction monitoring mode（MRM）.The calibration curve was linear over the concentration range of 0.5～40.0 μg/L（r0.999）.The recoveries ranged from 71.2% to 103% at the spiked level of 0.01,0.02,0.1,0.5 mg/kg.The relative standard deviations were between 3.7% and 9.8%.The limit of quantitation was 0.01 mg/kg.

关 键 词：高效液相色谱串联三重四极杆质谱 检测 饲料 β-受体激动剂 

分 类 号：O657.99[理学—分析化学]