甘蔗SPSⅢ内含子地高辛探针的制备及检测  

Preparation and Detection for Sugarcane SPSⅢ Intron Probes Labelled with Digoxgenin

作　　者：许云珍[1,2] 林荣华[1,2] 何文锦[1,2] 吴自明[1,2] 陈由强[1,2] 陈如凯[2] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108 [2]农业部甘蔗遗传改良重点开放实验室,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2010年第6期104-108,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(30871575);国家高技术研究发展计划"863"项目(SQ2007AA10XK140067);福建省自然科学基金资助项目(2009J05059)

摘　　要：以甘蔗SPS基因选择性剪接保留的内含子6,7,8片段为靶序列,利用PCR扩增技术分别制备出3种地高辛标记的SPS内含子探针:SPS-intron 6探针167 bp,SPS-intron 7探针93 bp和SPS-intron8探针175 bp,并通过斑点杂交实验表明这3种探针灵敏度高,都能检测到pg级的靶核酸,且特异性好,阴性对照实验显示无杂交信号.With sugarcane SPS Ⅲ gene alternative splicingintron retention 6,7,8 fragments as the three target sequences,PCR amplification technique prepared three SPSⅢ intron probes labelled with Digoxgenin：SPS-intron 6 probe 167 bp,SPS-intron 7 probe 93 bp and SPS-intron 8 probe 175 bp.Dot-blot hybridization indicated the sensitivity of the probes was high,detecting the pg level of the target nucleic acid,and the specificity was good,for the negative control showed non-hybrid signal.

关 键 词：甘蔗SPSⅢ 地高辛标记探针 斑点杂交 

分 类 号：Q946[生物学—植物学]