赤小豆总黄酮分光光度分析方法建立及全国不同产地药材含量测定  

UV-VIS Analysis Method of Total Flavonoids in Adzuki Bean and Determination of Different Origin Herbs

作　　者：卫莹芳[1] 闫婕[1] 王化东[1] 郭山山[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室,四川成都611137

出　　处：《时珍国医国药》2010年第11期2729-2731,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科技资源共享平台项目子课题(No.2005DKA21000)

摘　　要：目的建立一种测定赤小豆中总黄酮含量的方法,为赤小豆的质量控制提供科学依据。方法以芦丁为对照品,亚硝酸钠-硝酸铝比色法测定赤小豆总黄酮含量。结果线性方程为A=11.402C-0.004 9,相关系数r=0.999 9,平均加样回收率为100.55%,RSD为1.36%（n=6）。对全国11个省的37个不同产地的药材中总黄酮含量进行了测定,赤豆含量为0.76%~1.31%,赤小豆含量为0.84%~1.30%。结论该测定方法准确,重复性好,可用于赤小豆药材中总黄酮的含量测定。Objective To develop a UV-VIS analysis method for the determination of total flavonoids in adzuki bean,in order to provide a scientific basis for the quality control of adzuki bean.Methods With rutin as standard sample,sodium nitrite-aluminum nitrate colorimetric method was used to determine the content of total flavonoids in adzuki bean.Results The calibration curve of rutin was A=11.402C-0.004 9,r=0.999 9,and the average recovery was 100.55%,with RSD of 1.36%（n=6）.The content range of total flavonoids in Phaseolus angularis was from 0.76% to 1.31%,and content range in P.calcaratus was from 0.84% to 1.30%.Conclusion This detemination method is practical,reproducible and can be used for evaluating the quality of adzuki bean.

关 键 词：赤小豆 赤豆 总黄酮 芦丁 分光光度法 

分 类 号：R284.2[医药卫生—中药学]