川芎嗪对严重烧伤大鼠心肌细胞GRP94表达的影响  

Effects of Liqustazine on Expression Change of Glucose-regulated Protein 94 in the Cardiomyocytes of Severe Burn Rats

作　　者：王侃[1] 涂硕[1] 万慧芳[1] 余乐涵[1] 李华[1] 李国辉[1] 万福生[1] 

机构地区：[1]南昌大学医学院,江西南昌330006

出　　处：《时珍国医国药》2010年第11期2745-2747,共3页Lishizhen Medicine and Materia Medica Research

基　　金：江西省自然科学基金(No.0040054);江西省科技支撑计划重点项目(No.2006B0301307)

摘　　要：目的观察严重烧伤大鼠心肌细胞内质网应激蛋白表达的改变及川芎嗪对其的影响,探讨川芎嗪对严重烧伤后心肌损伤保护作用机制。方法建立大鼠30%Ⅲ度烫伤模型,酶联免疫法检测血浆中心肌肌钙蛋白T(cTnT)含量,放射免疫法检测血浆中TNF-α的含量,RT-PCR和免疫组化分析GRP94的表达。结果烧伤组大鼠伤后3 h血浆中cTnT含量即呈显著升高(P<0.01),心肌中GRP94 mRNA和蛋白表达于烧伤后3 h显著性升高,12 h达峰值,24 h还显著升高;Caspase-3活性在伤后3 h升高,12 h达高峰,48 h后仍显著高于对照组。川芎嗪治疗组GRP94的表达和Caspase-3活性较烧伤组均有显著性降低(P<0.05)。结论严重烧伤可引起心肌细胞内GRP94表达呈持续性升高,川芎嗪对烧伤后早期GRP94表达升高有较好的拮抗作用。Objective To observe the effects of liqustazine on expression change of glucose-regulated protein 94（GRP94） in the cardiomyocytes of severe burn rats.Methods Healthy adult Wistar rats were randomly divided into the control group（without burns）,the burn group（subjected to 30%TBSA III degree scalding） and the treatment group（treated with intraperitoneal injection of Liqustazine（20 mg/kg） immediately after scald injury）.The myocardial tissue samples were harvested at 1,3,6,12 and 24 hours post burn（PBH）.The cardiac troponin T（cTnT） and tumor necrosis factor alpha（TNF-α） contents in plasma were measured by enzyme linked immunosorbent assay method（ELISA） and radioimmuno assay（RIA） respectively,RT-PCR and immunohistochemistry were applied to monitor the gene expression of GRP94.Results the content of cTnT in plasma was significantly increased 3 hours post burn（P0.01）,the gene expression of GRP94 and caspase-3 activity 3 hours post burn reached the peak after 12 h,while the GRP94 expression in Liqustazine treatment group was dramatically decreased.Conclusion Severe burn can induce GRP94 expression increase in rat cardiomyocytes,Liqustazine has notable inhibitory effects on GRP94 expression increase in the cardiomyocytes during the early stage of postburn.

关 键 词：烧伤 川芎嗪 葡萄糖调节蛋白 心肌损伤 内质网应激 

分 类 号：R285.5[医药卫生—中药学]