对《中国药典》2005年版Ⅰ部栀子中栀子苷含量测定方法的探讨  

Discussion about Assay Method of Geniposide in Fructus Gardeniae in Chinese Pharmacopoeia(2005 Edition)Volume Ⅰ

作　　者：赵成城[1] 杨军宣[1] 施俊辉[1] 周子渝[1] 杨明[1,2] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《时珍国医国药》2010年第11期2793-2795,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家重大新药创制专项(No.2009ZX09103-393)

摘　　要：目的改进栀子药材中栀子苷的含量测定方法。方法比较了《中国药典》2005版栀子药材中栀子苷的含量测定方法和改进方法,用HPLC法测定栀子苷含量,流动相为乙腈-水（15∶85）,检测波长为238 nm。供试品用60%乙醇作为提取溶剂,超声处理,制得供试品溶液。结果改进方法所测定栀子中栀子苷含量高于药典法。栀子苷在0.075~0.60μg/ml范围内线性关系良好,r=0.999 2,平均加样回收率为100.45%,RSD=1.69%。结论该法灵敏、准确、重现性好,可用于栀子药材的质量控制,为改进栀子的含量测定方法提供依据。Objective To improve the method for determination of geniposide in Fructus Gardeniae.Methods The improved method of assay method of geniposide in Fructus Gardeniae was compared with the pharmacopoeia method.The content of geniposide was determined by HPLC at 238 nm detection wavelength with acetonitrile-water（15：85）as mobile phase.The sample was treated with 60% ethanol as solvent extraction,ultrasonic extraction,and the obtained sample solution was used for determination.Results The improved method for determination of geniposide was superior to the pharmacopoeia method.Geniposide in the range of 0.075 ~ 0.60μg/ml was linear,r=0.999 2,average recovery was 100.45%,RSD=1.69%.Conclusion This method is sensitive,accurate,reproducible,and can be used for the quality control of Fructus Gardeniae andsupplies reference evidence for the assay of Fructus Gardeniae.

关 键 词：栀子 栀子苷 含量测定 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]