全国不同产地及不同部位穿心莲中总内酯的含量测定  

Determination of Total Lactones in Different Areas and Parts of Andrographis

作　　者：郭山山[1] 卫莹芳[1] 闫婕[1] 王化东[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室,四川成都611137

出　　处：《时珍国医国药》2010年第11期2797-2799,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科技资源共享平台项目子课题(No.2005DKA21000)

摘　　要：目的测定全国8个省24个不同产地25个穿心莲样品及其中10个产地11个批次穿心莲不同部位中总内酯的含量。方法用二硝基苯甲酸和KOH试液显色,比色法在540 nm处测定穿心莲中总内酯的含量。结果不同产地及不同批次穿心莲样品中总内酯的含量在0.49%~2.76%之间,有显著差异。叶在1.82%~3.68%之间,茎在0.44%~1.04%之间,药材在1.27%~2.76%之间,穿心莲不同部位总内酯的含量有显著差异。结论该方法准确、可靠,为穿心莲的质量评价及质量控制提供依据。Objective To determine the contents of total lactones in 25 Andrographis samples from 24 origins or provinces and 11 batches of different parts among 10 origins.Methods With 2-nitrobenzoic acid and KOH test solution as color developing reagents,colorimetry method was used to determine the content of total lactones in Andrographis paniculata at the wavelength of 540 nm.Results The total lactones contents of Andrographis paniculata from different localities and batches were in the range of 0.49%～2.76%,and there was significant difference.Leaves were in the range of 1.82%～3.68%,stems were in the range of 0.44%～1.04%,medicines were in the range of 1.27%～2.76%,the total lactone content had significant difference in different parts of Andrographis paniculata.Conclusion This method is accurate and credible and it has provided the basis for the study of the quality assessment and control of Andrographis paniculata.

关 键 词：穿心莲 总内酯 比色法 

分 类 号：R284.2[医药卫生—中药学]