红景天酒中红景天苷含量的高效液相色谱测定  

Quantitative Determination of Salidroside in Hongjingtian Wine by HPLC

作　　者：杨道宾[1] 刘丽[1] 贾国夫[2] 刘素君[1] 

机构地区：[1]乐山师范学院化学与生命科学学院,四川乐山614004 [2]四川省草原科学研究院,四川成都611731

出　　处：《时珍国医国药》2010年第11期2799-2800,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技支撑计划项目(No.2007BAC18B04)

摘　　要：目的建立高效液相色谱法测定红景天酒中红景天苷含量的方法。方法色谱柱为Shim-pack VP-ODS C18（250mm×4.6 mm,5.0μm）,流动相甲醇-水（18∶82）,检测波长为278 nm,流速为1.0 ml/min,柱温为30℃。结果红景天苷进样量在0.072~1.296μg范围内与峰面积积分值呈良好的线性关系（r=0.999 9）,平均回收率为98.90（,RSD为1.32（。结论该法灵敏度高,操作简便,结果准确可靠,可用于红景天酒中红景天苷含量的测定及其质量控制与评价。Objective To establish an HPLC method for the quantitative determination of salidroside in Hongjingtian wine.Methods The samples were separated on a Shim-pack VP-ODS C18（250 mm×4.6 mm,5.0 μm） column with the mobile phase of methanol-water（18：82） in the flow rate of 1.0 ml·min-1.The detection wavelength was set at 278 nm.column temperature was 30 ℃.Results The linear range of salidroside was 0.072～1.296 μg（r=0.999 9）.The average recovery of salidroside was 98.90 and the RSD was 1.32%（n=5）.Conclusion This method is sensitive,simple and accurate,which can be used to evaluate the quality control of Hongjingtian wine.

关 键 词：红景天酒 红景天苷 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]