复叶耳蕨提取物体外抗HIV-1整合酶活性的研究  

Inhibitory Effect of Different Extract Parts from Arachniodes exilis on HIV-1 IN Activities

作　　者：李辉敏[1] 殷嫦嫦[1] 吴家忠[1] 张磊[1] 

机构地区：[1]九江学院基础医学院,江西九江332000

出　　处：《时珍国医国药》2010年第11期2827-2828,共2页Lishizhen Medicine and Materia Medica Research

基　　金：江西省自然科学基金(No.2007GQY0114)

摘　　要：目的对鳞毛蕨科复叶耳蕨属植物复叶耳蕨不同提取部位体外抗HIV-1整合酶活性进行研究。方法在体外无细胞系统内采用酶联免疫吸附测定HIV-1整和酶的半数抑制浓度(IC50)。结果复叶耳蕨醋酸乙酯提取物、正丁醇提取物抑制HIV-1整合酶的半数有效浓度IC50分别为44.30,16.02μg/ml,水提取物抑制HIV-1整合酶的半数有效浓度IC>100μg/ml。结论复叶耳蕨醋酸乙酯提取物与正丁醇提取物具有体外抑制HIV-1整合酶活性。Objective To evaluate the anti-HIV-1IN activities of the different extract parts from Arachniodes exilis Ching.Methods HIV-1 IN reverse transcriptase,protease and integrase were detected by enzyme –linked inmunosorbent assay.Results In the experiment of anti-HIV IN,the IC50 of the n-butanol extract acetic ether extract were 44.30,16.02μg/ml,respectively.The IC50 of the aqueous extract was higher than 100 μg/ml.Conclusion The n-butanol extract,acetic ether extract from Arachniodes exilis Ching have the inhibitory effect on HIV-1 IN activities.

关 键 词：复叶耳蕨提取物 抗HIV-I整合酶活性 

分 类 号：R962[医药卫生—药理学]