HPLC测定蒲公英中槲皮素含量的研究  

Determination of Quercetin Content in Dandelion by HPLC

作　　者：肖奇志[1] 

机构地区：[1]贵阳护理职业学院,贵州贵阳550003

出　　处：《安徽农业科学》2010年第30期16871-16872,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立蒲公英中槲皮素含量的测定方法。[方法]采用高效液相色谱法,AgilentC18柱(150.0mm×4.6mm,5.0μm),流动相为甲醇-乙腈(63∶26),紫外检测波长为370nm。[结果]槲皮素在0.1～1.0mg/ml范围内线性关系良好,平均回收率为98.37%。[结论]该方法分离好、快速、重复性好,可作为蒲公英的质量控制方法。[Objective]The research aimed to establish a method for determining the content of quercetin in dandelion.[Method]The content of quercetin in dandelion was determined by HPLC.The analysis was performed on Agilent C18 column（150.0 mm×4.6 mm,5.0 μm）,with the mixture of methanol and acetonitrile（63：26） as mobile phase.The detection wave length was 370 nm.[Result]The linear range of quercetin was 0.1-1.0 mg/ml.The average recovery was 98.37%.[Conclusion]The method is simple,sensitive and accurate for HPLC to control the quality of dandelion.

关 键 词：蒲公英 槲皮素 高效液相色谱法 

分 类 号：S682.11[农业科学—观赏园艺]