嵌段共聚物改性表面对蛋白质吸附和细胞黏附的调控  

Regulation of protein adsorption and cell adhesion on surfaces modified by block copolymer brushes

作　　者：张燕霞[1] 于谦[1,2] 周峰[1,2] 徐亚骏[2] 陈红[2] 

机构地区：[1]武汉理工大学材料科学与工程学院,武汉430070 [2]苏州大学材料与化学化工学部,苏州215006

出　　处：《科学通报》2010年第29期2808-2814,共7页Chinese Science Bulletin

基　　金：国家自然科学基金资助项目(20634030,20974086)

摘　　要：通过表面引发原子转移自由基聚合的方法制备了聚甲基丙烯酸寡聚乙二醇酯-嵌段-聚苯乙烯(POEGMA-b-PS)改性的硅表面.通过控制反应时间可以对两种组分的长度进行调控.结果发现:POEGMA和PS相对长度的变化会引起改性表面的化学组成和微结构发生改变,进而导致表面对蛋白质吸附和细胞黏附情况的改变.随着PS链段相对长度的增加,改性表面的疏水性增强,对纤维蛋白原的吸附量增加,同时成纤维细胞在表面的黏附和铺展情况转好.当PS链段相对较短时,POEGMA-b-PS改性表面既能排斥蛋白质的非特异性吸附,同时又能够支持细胞在表面的黏附生长,这在组织工程领域有着潜在的应用.Poly oligo（ethylene glycol） methacrylate-block-polystyrene （POEGMA-b-PS） brushes were prepared via surface-initiated consecutive atom-transfer radical polymerization on initiator-immobilized silicon.The block length of the two blocks was modulated by adjusting reaction time.The characterization results indicated that the variation of relative length of POEGMA and PS would result in the change of chemical composition and microstructure,and furthermore the change of protein adsorption and cell adhesion on the modified surfaces.As the length of PS block increased,the modified surface became more hydrophobic and the adsorption of fibrinogen increased;at the same time the adhesion and spreading of fibroblasts turned better.The POEGMA-b-PS modified surface could both resist non-specific protein resistance and promote cell adhesion when the length of PS block was shorter,which may have potential applications in tissue engineering.

关 键 词：聚甲基丙烯酸寡 聚乙二醇酯 聚苯乙烯 嵌段共聚物 蛋白质吸附 细胞黏附 

分 类 号：R318.08[医药卫生—生物医学工程]