干燥综合征患者肾组织中人B细胞激活因子的表达  

Expression of B lymphocyte stimulator in the renal tissues of patients with primary Sjgren syndrome

作　　者：任红[1] 王伟铭[1] 陈晓农[1] 张文[1] 潘晓霞[1] 陈楠[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院肾脏科,上海200025

出　　处：《肾脏病与透析肾移植杂志》2010年第5期421-423,429,共4页Chinese Journal of Nephrology,Dialysis & Transplantation

基　　金：国家自然基金(30670972)(81070560);上海市重点学科建设基金(T0201);上海市卫生局重点学科建设基金(05Ⅲ001);上海市卫生局重点课题(2003ZD002)

摘　　要：目的:检测人B细胞激活因子(B lymphocyte stimulator,BlyS)在原发性干燥综合征(primary Sjgrensyndrome,pSS)患者肾组织中的表达,并比较在高丙种球蛋白(IgG)与丙种球蛋白正常两组患者中BlyS表达的差异。方法:选取上海市瑞金医院肾脏科住院诊断为pSS,且合并肾脏损害的34例患者的肾活检标本,按血清IgG水平,分为高IgG组(IgG≥1660mg/dl)和正常IgG组(IgG<1660mg/dl),应用免疫荧光法观察BlyS在肾组织中的表达,以5例正常肾脏组织作为对照,采用one way ANOVA分析法比较在高IgG与正常IgG两组患者中BlyS表达的差异。结果:免疫荧光结果显示:(1)BlyS在正常肾脏组织中,无或仅少量BlyS表达;而在pSS患者肾脏组织中可见浸润的炎性细胞特异性荧光亮染,BlyS主要表达细胞膜上。在肾脏小管间质区和肾小球中浸润的炎细胞中圴有BlyS的表达。(2)高IgG组与正常IgG两组患者肾脏组织BlyS表达的差异以荧光强度反映BlyS表达差异,发现正常IgG组患者肾组织BlyS表达高于高IgG组(P<0.05)。结论:pSS患者肾脏组织BlyS表达显著高于正常对照,其不仅表达在肾小管间质浸润的炎性细胞表面,而且在肾小球炎性细胞中也有表达。Objective：To investigate the expression of B lymphocyte stimulator （BIyS） in the renal tissues of patients with primary SjiSgren syndrome （pSS）. Methodology：All patients, who diagnosed as pSS and admitted to our hospital, were retrospectively analyzed. Renal biopsy was performed on 34 patients. The expression of BlyS in the renal tissues was detected by indirect immunofluorescence. The renal tissues from 5 healthy people were selected as normal controls. One way ANOVA analysis was used for the statistics. Results： Immunofluorescence revealed that BlyS was expressed in the inflammation cells distributed in both renal interstitium and glomerulum, but there was hardly no BlyS expression in normal controls. The expression density of BlyS in patients with hypergammaglobulin was significantly higher than that in patients with normal serum IgG （ P 〈 0. 05 ）. Conclusion： BIyS was expressed in the inflammation cells distributed in both renal interstitium and glomerulum The expression of BlyS in the renal tissues of pSS patients was significantly higher than that of normal controls. The level of serum IgG affected the expression level of BIyS in renal biopsied sections.

关 键 词：干燥综合征 高丙种球蛋白血症 B细胞激活因子 

分 类 号：R593.2[医药卫生—内科学]