我国不同品系实验室小鼠肝螺杆菌感染状况调查  

Investigation of Helicobacter hepaticus infection in various species of mice in China

作　　者：季尚玮[1] 王颂[2] 王江滨[1] 张永贵[1] 

机构地区：[1]吉林省消化系疾病重点实验室,吉林大学中日联谊医院消化科,130033 [2]吉林大学第二医院泌尿外科

出　　处：《中华消化杂志》2010年第9期597-601,共5页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目（30670300）;吉林大学研究生985创新项目（20080227）

摘　　要：目的 调查我国不同地域不同品系实验室小鼠中是否存在肝螺杆菌（Hh）感染,为进一步探讨Hh在小鼠慢性肝炎、肝细胞癌以及下消化道肿瘤中的作用奠定基础.方法 收集国内不同地域的114只不同品系小鼠,其中C57BL/6小鼠39只,BABL/C小鼠43只,SCID小鼠14只,C3H小鼠18只.分别用ELISA法检测小鼠血清Hh抗体和粪便Hh抗原;PCR法检测粪便螺杆菌菌属特异性16SrRNA基因和Hh特异性16SrRNA基因;粪便螺杆菌分离、培养及鉴定等方法分析Hh在小鼠中的感染状况.上述方法中有一项阳性即判断为存在Hh感染.结果 在114只小鼠中有25只感染了螺杆菌（21.9%）,其中44.0%（11/25）为Hh感染,并以SCID小鼠和C3H小鼠Hh感染率较高.同时,PCR检测结果显示,在其余56.0%（14/25）的螺杆菌感染小鼠中发现存在其他螺杆菌感染.结论 来自我国不同地域不同品系实验室小鼠中除存在Hh感染外,可能还存在其他螺杆菌的感染.Objective To investigate the prevalence of Helicobacter hepaticus （H. hepaticus）infection in various species of mice from different regions of China in order to find the role of H. hepaticus in development of hepatitis, liver cancer and tumors in lower digestive tract in mice.Methods One hundred and fourteen mice, including C57BL/6 mice （n= 39）, BABL/C mice （n=45）,SCID mice （n=14） and C3H mice （n=18）, were collected from different regions of China. The serum anti-H, hepaticus-IgG and fecal H. hepaticus antigen were determined by using ELISA. Polymerase chain reaction analysis （PCR） was used to screen Helicobacter genus-specific 16SrRNA and H. hepaticus species-specific 16SrRNA. The feces were cultured and identitied for Helicobacter infection in 114 mice. The H. he paticus infection was identified as one of above tests being positive.Results Of 114 mice, 25 （21.9%） mice were infected with Helicobacter species. The mice infected with H. hepaticus accounted for 44. 0% （11/25） with SCID and C3H mice in high prevelence.Meanwhile, the PCR examination revealed that the rest 56.0% （14/25） mice were infected with other Helicobacter species. Conclusion Besides H. hepaticus infection, the other Helicobacter species infections are also existed in China.

关 键 词：肝螺杆菌 感染 小鼠 

分 类 号：R686[医药卫生—骨科学]