羟基磷灰石对海藻酸钙水凝胶的制备及性能的影响  

Effect of Hydroxyapatite on the Preparation and Properties of Alginate Hydrogel

作　　者：孙静[1] 肖玉梅[1] 范红松[1] 张兴栋[1] 

机构地区：[1]四川大学国家生物医学材料工程技术研究中心,成都610064

出　　处：《无机材料学报》2010年第10期1087-1091,共5页Journal of Inorganic Materials

基　　金：国家重点基础研究发展计划(973计划)(2005CB623901)~~

摘　　要：采用具有良好生物相容性的羟基磷灰石(HA)作为钙源,开发了一种新型的HA-GDL交联体系制备海藻酸钙水凝胶.通过调节海藻酸钠溶液的浓度CSA和钙离子与海藻酸钠中羧基的摩尔比f,分别制备CSA=2%、2.5%、3%,f=0.27、0.36、0.54的一系列水凝胶.以CaCO3-GDL体系作为对照,系统比较了两种体系制得的水凝胶在凝胶化时间、凝胶形貌、力学性能以及溶胀收缩等方面的性能.结果显示,相同凝胶条件下,采用HA-GDL体系制得的海藻酸钙水凝胶具有较快的凝胶化速率,较为均一的凝胶结构,较强的力学性能和较低的溶胀率.将海藻酸钙凝胶与软骨细胞复合进行体外培养,结果显示该体系具有良好的细胞相容性,从而说明该材料体系可能是一种良好的软骨组织工程支架材料.A novel crosslingking system,hydroxyapatite-D-glucono-δ-lactone（HA-GDL） was developed to prepare alginate hydrogel,in which calcium ions can be released to initiate gelation.A series of hydrogels were prepared by adjusting the sodium alginate（SA） concentration and the molar ratio of calcium ion in HA to carboxyl of SA.Gelation time,surface morphology,mechanical property and swelling capacity of hydrogels crosslinked by HA-GDL system were characterized and compared with that crosslinked by the calcium carbonate-D-glucono-δ-lactone（CaCO3-GDL） system.The results show that under the same conditions,the hydrogels obtained from the HA-GDL system have faster gelation rate,more uniform structure,stronger mechanical strength and lower swelling ratio compared with that obtained from CaCO3-GDL system.Chondrocytes of newborn rabbit are embedded in alginate hydrogels to construct tissue engineered cartilage in vitro.Primary results show that the alginate hydrogels prepared with HA-GDL system have good cell compatibility and are with potential to be used as a scaffold for cartilage repair.

关 键 词：海藻酸 水凝胶 羟基磷灰石 碳酸钙 组织工程 

分 类 号：R318[医药卫生—生物医学工程]