17β-雌二醇激发鲻早期精子发生的作用机制  

Stimulating mechanism of estradiol-17β in early spermatogenesis of grey mullet,Mugil cephalus

作　　者：宋海霞[1] 翁幼竹[1] 方永强[1] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《中国水产科学》2010年第6期1192-1199,共8页Journal of Fishery Sciences of China

基　　金：国家海洋局第三海洋研究所基本科研业务费专项资金资助项目(海三科2008011)

摘　　要：将雄性幼鲻(Mugil cephalus)分为4组,分别投喂含17β-雌二醇饲料组、含三苯氧胺(Tamoxifen)饲料组、含17β-雌二醇和三苯氧胺(Tamoxifen)混合饲料,以及对照组,旨在进一步研究17β-雌二醇对幼鲻精子发生的作用机制。同时,采用组织学和免疫组织化学方法对17β-雌二醇影响鲻精巢精子发生及其作用机制进行分析,结果表明,雌激素明显促进鲻精原细胞有丝分裂及增殖和精子发生;Tamoxifen组与混合组于实验后30d取材观察,两组精巢切片均显示,雄性鲻精巢发育停滞,精原细胞坏死、凋亡,Sertoli’s细胞形态结构发生改变、数量下降。这进一步证实雌二醇激发精子发生必须经其受体的介导。雌激素受体(ER)在精巢的免疫组织化学定位揭示,ER在雌激素组免疫活性最高,对照组次之,混合投喂组免疫活性非常弱,而Tamoxifen组则显免疫阴性反应,这为雌激素对鲻精巢的精子发生起着关键作用的论点提供可靠确凿的新证据,同时表明在鲻早期精子发生中,一旦雌激素不足或缺失,精巢的发育质量和生精能力就会受到严重的影响。Using histology and immunohistochemical methods,the effects and action mechanism of estradiol-17β（E2） on early spermatogenesis of grey mullet,Mugil cephalus,were further investigated.Healthy male juvenile grey mullets were randomly divided into four groups：which were fed with estradiol-17β,tamoxifen,estradiol-17β ＋ Tamoxifen and basic diet（control）,respectively,for 45 days,then all experimental fish were fed with basic diet for 15days instead,and the experiment were finished.The results showed E2 could hasten cell division,proliferation and spermatogenesis obviously.But the Tamoxifen group and the mixed group showed the arrest of development,cell necrosis and apoptosis of spermatogonia.Meanwhile,the morphology and quantity were changed in Sertoli＇s cells after feeding 30 days,which further verified E2 stimulated spermatogenesis by its receptor mediated.The Estrogen receptor（ER） localization in the early testis of grey mullet showed the immunoreactivity of ER was the highest in E2 group,the control group took place secondly,the mixed group was the worst,and the Tam group showed immuno-negative.So,the immunohistochemical results futhur proved that E2 played a crucial role on inducing spermatogenesis,when it was deficiency or absence,and the development quality and spermatogenic activity would be affected seriously.

关 键 词：鲻 精子发生 17Β-雌二醇 三苯氧胺 

分 类 号：Q959[生物学—动物学] S96[农业科学—水产养殖]