不同浓度人脐带间充质干细胞移植修复大鼠脊髓损伤的实验研究  

Effect of different concentration of human umbilical cord mesenchymal stem cells in experimental spinal cord injury in rats

作　　者：赵鹏[1] 冯世庆[1] 王颖[1] 魏志坚[1] 焦新旭[1] 

机构地区：[1]天津医科大学总医院骨科,天津市和平区鞍山道154号300052

出　　处：《中国矫形外科杂志》2010年第21期1817-1821,共5页Orthopedic Journal of China

基　　金：国家自然科学基金(编号:30872603);天津市卫生局科技重点基金(编号:07KG2);天津市卫生局科研基金(编号:09KZ104)

摘　　要：[目的]比较不同浓度人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,HUCMSCs)移植修复大鼠脊髓损伤(spinal cord injury,SCI)的疗效。[方法]利用NYU Impactor model-Ⅱ打击器制作大鼠T10 SCI模型,分组为:80只Wistar大鼠随机分为DMEM实验对照组(A组)、1×104HUCMSCs移植组(B组)、1×105HUCMSCs移植组(C组)和1×106HUCMSCs移植组(D组),每组20只。对各组实验动物SCI后肢体功能恢复情况进行行为学评分(BBB评分),并以HE染色和免疫组化染色及生物素葡聚糖胺(BDA)顺行示踪皮质脊髓束(corticospinal tract,CST)染色观察SCI处轴突再生情况。[结果]与实验对照组相比,HUCMSCs移植能促进SCI后轴突再生,改善大鼠后肢功能恢复。D组优于其他组,各组间BBB评分差异有统计学意义(P<0.05);NF-200免疫荧光染色累积光密度各组间差异均有统计学意义(P<0.05)。[结论]HUCMSCs在体内可向神经元及神经胶质细胞分化,促进SCI后轴突再生和肢体功能恢复。[Objective]To compare the effects of different concentration of HUCMSCs in rats after SCI.[Methods]The rat SCI model was made by NYU Impactor-Ⅱ at T10.Eighty rats were randomly divided into four groups： DMEM control group（group A）,HUCMSCs grafted 1×104 post injury（group B）,HUCMSCs grafted 1×105 post injury（group C） and HUCMSCs grafted 1×106 post injury（group D）.The hindlimb functional recovery of rats in each group was evaluated by Basso Beattie Bresnahan（BBB） score,HE,immunohistochemistry staining and Biotinylated Dextran Amine（BDA） antergrade tracing.[Results]Compared with the control group,grafted HUCMSCs showed larger effect on functional recovery,especially for group D.Statistic difference existed among the four groups（P0.05）.[Conclusion]HUCMSCs differentiates into neurons and glial cells in vivo,could promote axonal regeneration and functional recovery after SCI.

关 键 词：不同浓度 脊髓损伤 细胞移植 人脐带间充质干细胞 

分 类 号：R651.2[医药卫生—外科学]