人心肌肌钙蛋白I胶体金免疫层析试纸条的研制  

Development of colloidal gold immunochromatographic test strip for human cardiac troponin I

作　　者：郝建秀[1] 张春明[1] 王沂[1] 宋娜玲[1] 贺欣[1] 刘鉴峰[1] 王德芝[1] 

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所天津市分子核医学重点实验室,天津300192

出　　处：《生物医学工程与临床》2010年第6期546-549,共4页Biomedical Engineering and Clinical Medicine

基　　金：天津市自然科学基金重点项目(08JCZDJC16200)

摘　　要：目的建立一种简便实用胶体金免疫层析检测方法,用于人心肌肌钙蛋白I(cTnI)的快速检测。方法采用柠檬酸三钠还原法制备胶体金,以鼠源抗cTnI单克隆抗体进行胶体金标记,以兔源抗cTnI多克隆抗体固定于硝酸纤维素膜作为捕获抗体,采用免疫层析技术制备快速检测cTnI胶体金免疫层析检测试纸条,并与国外生产的胶体金试剂盒作对比。结果该试纸条检测范围质量浓度为5 ng/mL～1μg/mL;灵敏度质量浓度为5 ng/mL;特异性:与肌红蛋白、肌酸磷酸激酶同工酶、cTnT、cTnC无交叉反应;与国外胶体金试纸条检测结果比较阳性符合率为96.4%,阴性符合率为100.0%。结论成功建立cTnI胶体金免疫层析检测方法,该方法灵敏度高,特异性强,检测速度快,适于急性心肌梗死的早期诊断及科研工作需要。Objective To develop the colloidal gold immunochromatographic assay for rapid detection of human cardiac troponin I（cTnI）. Methods The mouse monoclonal antibody cTnI was conjugated with colloidal gold particles prepared by sodium citrate reduction method, the rabbit polyclonal antibody was immobilized on nitrocellulose filter and prepared a colloidal gold immunochromatographic strip by immunity chromatography technology. The result of strip was compared with colloidal gold kit of GiBco-BRL company. Results The detection range of colloidal gold immunochromatographic strip was 5 ng/mL - 1 p,g/mL, the sensitivity was 5 ng/mL, and specificity was no cross-reaction with myoglobin（Mb）, creatine kinase MB（CK-MB）, cTnT and cTnC. Compared with colloidal gold kit of GiBco-BRL company, the positive coincidence rate was 96.4 % and the negative coincidence rate was 100.0 %. Conclusion It is demonstrated that colloidal gold immunochrematographic assay exhibit high sensitivity and specificity to detect cTnI, and it is rapid, economical and simple, without requirement of complicated equipment. It is suitable for early diagnosis of acute myocardial infarction and scientific research.

关 键 词：心肌肌钙蛋白Ⅰ 胶体金 免疫层析技术 实验室检查 急性心肌梗死 

分 类 号：R541[医药卫生—心血管疾病] R446.61[医药卫生—内科学]