Effect of EGF1 peptides in directing nanoparticles to thrombi  

作　　者：MEI Heng PANG ZhiQing HU Yu SHI Wei WANG HuaFang DENG Jun GUO Tao JIANG XinGuo 

机构地区：[1]Institute of Hematology, Union Hospital, Tongji Medical College, Huaz~ong University of Science & Technology, Wuhan 430022, China [2]Key Laboratory of Targeted Biotherapy, Ministry of Education, Wuhan 430022, China [3]Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai 200032, China

出　　处：《Chinese Science Bulletin》2010年第30期3424-3429,共6页

基　　金：supported by National Basic Research Program of China (2007CB935803);the National Natural Science Foundation of China (30825018)

摘　　要：In order to examine the effect of EGF1 peptides in directing nanoparticles to thrombi,the synthesized EGF1 peptide was conjugated to poly (ethyleneglycol)-poly (lactide) (PEG-PLA) nanoparticles (NP) to form EGF1-NP. A lipophilic fluorescent dye,coumarin-6,was incorporated into EGF1-NP so as to detect its loading and release capacity. The binding ability of EGF1-NP with TF-expressing cells was shown to be significantly higher than that of the non-conjugated NP. Following an intravenous administration,fluorescence was distributed along the vessel wall of the thrombosis regions in the model rats injected with coumarin-6-loaded EGF1-NP. The in vitro and in vivo results suggest that EGF1-NP is a promising drug delivery system for targeting cerebral thrombi.In order to examine the effect of EGF1 peptides in directing nanoparticles to thrombi, the synthesized EGF1 peptide was conjugated to poly （ethyleneglycol）-poly （lactide） （PEG-PLA） nanoparticles （NP） to form EGF1-NP. A lipophilic fluorescent dye, coumarin-6, was incorporated into EGF1-NP so as to detect its loading and release capacity. The binding ability of EGF1-NP with TF-expressing cells was shown to be significantly higher than that of the non-conjugated NP. Following an intravenous admini- stration, fluorescence was distributed along the vessel wall of the thrombosis regions in the model rats injected with coumarin-6-loaded EGFt-NP. The in vitro and in vivo results suggest that EGFt-NP is a promising drug detivery system for targeting cerebral thrombi.

关 键 词：纳米粒子 血栓形成 肽 聚乙二醇 结合能力 香豆素类 荧光染料 大鼠模型 

分 类 号：Q592.1[生物学—生物化学] TB383[一般工业技术—材料科学与工程]