The PPR protein PDM1 is involved in the processing of rpoA pre-mRNA in Arabidopsis thaliana  

作　　者：WU Hao ZHANG LiXin 

机构地区：[1]School of Life Sciences, Lanzhou University, Lanzhou 730000, China [2]Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

出　　处：《Chinese Science Bulletin》2010年第30期3485-3489,共5页

基　　金：supported by the National Basic Research Program of China (2006CB910300);the National Natural Science Foundation of China (30725003)

摘　　要：Pentatricopeptide repeat (PPR) proteins,containing tandem repeats of degenerate 35 amino acid motifs,are important for post-transcriptional chloroplast gene expression. In this study,we report the characterization of a pigment-deficient mutant 1 (pdm1) in Arabidopsis,which displays the albino phenotype. PDM1 contains 5 PPR motifs followed by a PLS domain. The levels of plastid-encoded polymerase-dependent chloroplast genes were reduced dramatically,whereas those of nucleus-encoded polymerase-dependent chloroplast genes increased in the mutant. In addition,the pattern of rpoA pre-mRNA was altered and the rpoA transcript was absent in pdm1. Thus,these results suggest that PDM1 is required for processing of rpoA pre-mRNA in Arabidop-sis.Pentatricopeptide repeat （PPR） proteins, containing tandem repeats of degenerate 35 amino acid motifs, are important for post-transcriptional chloroplast gene expression. In this study, we report the characterization of a pigment-deficient mutant 1 （pdml） in Arabidopsis, which displays the albino phenotype. PDM1 contains 5 PPR motifs followed by a PLS domain. The levels of plastid-encoded polymerase-dependent chloroplast genes were reduced dramatically, whereas those of nucleus-encoded poly- merase-dependent chloroplast genes increased in the mutant. In addition, the pattern of rpoA pre-mRNA was altered and the rpoA transcript was absent in pdml. Thus, these results suggest that PDM1 is required for processing of rpoA pre-mRNA in Arabidopsis.

关 键 词：基因表达 拟南芥 PR蛋白 前处理 叶绿体基因 缺失突变体 串联重复序列 薪酬水平 

分 类 号：Q78[生物学—分子生物学] Q943