NgR在实验性自身免疫性脑脊髓炎中的表达及NgR（310）ecto-Fc的干预作用  

Expression of NgR in experimental allergic encephalomyelitis （EAE） and intervention of NgR （310）ecto-Fc in EAE

作　　者：高聪[1] 占婷婷[1] 谢富华[1] 林美容[1] 林哲聪[1] 

机构地区：[1]广州医学院附属第二医院神经内科,广州510260

出　　处：《中华神经医学杂志》2010年第11期1086-1089,共4页Chinese Journal of Neuromedicine

基　　金：广东省自然科学基金（8151018201000036）;广东省科技计划基金（2009B030801363）

摘　　要：目的 探讨NgR（310）ecto-Fc在实验性自身免疫性脑脊髓炎（EAE）中的干预作用及其作用机制.方法 90只Lewis大鼠按随机数字表法分为3组,即A组（未治疗）、B组[开始免疫后第1天即给予NgR（310）ecto-Fc进行治疗]及C组[刚发病即给予NgR（310）ecto-Fc进行治疗],每组各30只.采用免疫组化法观察各组实验大鼠脊髓组织中NgR的表达变化及阳性细胞计数,同时观察干预前后临床评分变化.结果 免疫后第15天、第25天B、C两组平均临床评分（3.020±1.017,1.365±0.127;2.853±0.958,1.275±0.092）较A组（4.476±1.525,1.894±0.135）明显降低,比较差异有统计学意义（P＜0.05）;B组和C组之间平均临床评分比较差异无统计学意义（P＞0.05）.所有实验大鼠的脊髓组织中均可见NgR的表达;免疫后第15天、第25天B、C两组NgR阳性细胞计数（79.07±10.31,45.89±4.77;70.47±7.40,40.63±4.15）较A组（101.12±11.97,62.95±7.11）明显减少,比较差异有统计学意义（P＜0.05）;B组与C组NgR阳性细胞计数比较差异无统计学意义（P＞0.05）.结论 NgR（310）ecto-Fc能够减轻EAE模型的临床症状,可能是其通过抑制NgR的表达,从而同时封闭3个髓磷脂相关抑制因子（Nogo-A、MAG及OMgp）的作用,到达促进轴突再生的作用.Objective To investigate the role and the mechanism of NgR （310）ecto-Fc in experimental allergic encephalomyelitis （EAE）. Methods EAE models were successfully induced in 90 Lewis rats and equally randomized into group A （without treatment）, group B （giving NgR（310）ecto-Fc treatment 1 d after the success of model making） and group C （giving NgR（310）ecto-Fc treatment right after onset of the disease）. The clinical scores, pathological changes of the animals were observed and compared before and after treatment. Changes of NgR expression and counts of NgR（310）ecto-Fc positive cells in the myeloid tissue were tested by immunohistochemistry before and after treatment.Results Clinical scores in group B （3.020±1.017, 1.365±0.127） and group C （2.853±0.958, 1.275±0.092） were significantly lower than those in group A （4.476± 1.525, 1.894＋0.135） on the 15th and 25th d of success of model making （P〈0.05）, while no significant differences on the clinical scores were noted between group B and group C. NgR expression was observed in the myeloid tissue of all groups; the counts of NgR（310）ecto-Fc positive cells in the myeloid tissue in group B （79.07± 10.31, 45.89±4.77） and group C （70.47±7.40, 40.63±4.15） were obviously decreased as compared with those in group A （101.12±11.97, 62.95±7.11） on the 15th and 25th d of success of model making （P〈0.05）; while no significant differences on the counts of NgR （310）ecto-Fc positive cells were noted between group B and group C （P〉0.05）. Conclusion NgR （310）ecto-Fc can alleviate the clinical symptoms of EAE by suppressing the expression of NgR, leading to no activation of myelin-related inhibitory factor （Nogo-A, MAG and OMgp）, and inducing the growth of axons in EAE.

关 键 词：实验性变态反应性脑脊髓炎 NgR(310)ecto—Fc Nogo-66(NgR) 

分 类 号：R686[医药卫生—骨科学]