腰椎间关节软骨多序列MRI与大体标本对照实验研究  

Comparison between pig lumbar zypapophyseal joint cartilage acquired from multiple magnetic resonance image sequences and gross specimens

作　　者：廖红荔[1] 郁文明[2] 王维[1] 廖云杰[1] 

机构地区：[1]中南大学湘雅三医院放射科,长沙410013 [2]湖南师范大学医学院医学影像教研室,长沙410013

出　　处：《中南大学学报（医学版）》2010年第10期1064-1072,共9页Journal of Central South University ：Medical Science

基　　金：湖南省教育厅科研项目(09C628)~~

摘　　要：目的:通过对猪腰椎间关节软骨1.5T磁共振(magnetic resonance,MR)多序列成像与大体标本对照的实验性研究,评估MR成像(MR image,MRI)显示腰椎间关节软骨的能力与限度。方法:用Siemens1.5T超导磁共振对6条新鲜离体成年猪腰椎行椎间关节矢状面扫描。扫描序列包括:快速自旋回波T1加权序列(fast spin echo T1-weighted imaging,FSE T1WI)、快速自旋回波T2加权序列(fast spinecho T2-weighted imaging,FSE T2WI)、脂肪抑制质子加权序列(fat saturation proton density-weighted imaging,FS PDWI)、不同反转角的三维快速小角度激发成像序列(three-dimensional fast low angle shot imaging,3D-FLASH)以及不同反转角的水激发三维快速小角度激发成像序列(water excitation three-dimensional fast lowangle shot imaging,WE3D-FLASH)。扫描结束后将标本深低温冷冻,沿椎间关节矢状面正中层面切开,对照观察椎间关节矢状面正中层面大体标本与MRI图像。取6对L3/L4椎间关节矢状面正中层面各序列图像测量并计算软骨的信噪比(signal-noise ratio,SNR)以及软骨与骨皮质、软骨与骨髓、软骨与生理盐水的对比噪声比(contrast-noise ratio,CNR)。以游标卡尺测量6对L3/L4椎间关节软骨矢状面正中层面中心的厚度,与MRI所测量软骨厚度对照。结果:3D-FLASH(FA20°)及WE3D-FLASH(FA20°)序列显示腰椎间关节软骨及周围结构优于其他研究序列,相对FS PDWI序列能更真实地反映腰椎间关节软骨厚度。(1)4种不同反转角(FA10°,FA20°,FA30°及FA40°)3D-FLASH序列比较:3D-FLASH(FA20°)序列图像软骨SNR、软骨与骨皮质CNR最高,与其他3种3D-FLASH序列比较差异有统计学意义(P<0.05),软骨与骨髓CNR、软骨与生理盐水CNR亦较高。(2)4种不同反转角(FA10°,FA20°,FA30°及FA40°)WE3D-FLASH序列比较:WE3D-FLASH(FA20°)序列图像软骨SNR、软骨与骨皮质CNR、软骨与骨髓CNR最高,与其他3种WE3D-FLASH序列比较差异有统计学意义(P<0.05),软骨与生理�Objective To evaluate the capability and limitation of magnetic resonance image（MRI）for lumbar zygapophyseal joint cartilage through comparing pig lumbar zygapophyseal joint cartilage acquired from multiple MRI sequences of a 1.5 Tesla MR and gross specimens.Methods Six fresh lumbar spines from adult pigs were sagittaly scanned by Siemens 1.5 Tesla MR. The scan sequences included fast spin echo T1-weighted imaging （FSE T1WI）,fast spin echo T2-weighted imaging （FSE T2 weighted T2WI）,fat saturation proton density-weighted imaging （FS PDWI）,3-dimensional fast low angle shot imaging （3D-FLASH）,and water excitation 3-dimensional fast low angle shot imaging （WE 3D-FLASH）. Each scan sequence acquired images from the same layer. The signal-noise ratio （SNR） for articular cartilage,contrast-noise ratio （CNR） for cartilage versus bone cortex,cartilage versus bone marrow,and cartilage versus saline were calculated.Right after the scanning,the lumbar spines were snap-frozen,incised sagittally along the midline lumbar zypapophyseal joints,and photographed to compare the gross specimens with corresponding MRIs. The thickness of sagittal midline center of 6 pairs of lumbar（L3/L4）zypapophyseal joint cartilage was measured by vernier caliper. The thickness of the back ventral articular cartilage was added and then compared with corresponding MR images.Results 3D-FLASH（FA 20°）and WE 3D-FLASH（FA 20°） sequences had significant advantages compared with other sequences in imaging lumbar zypapophyseal joint cartilage,and were mostly close to the real thickness.（1） Comparison of the 4 flip angle （FA10°,FA 20°,FA 30°,and FA40°） 3D-FLASH sequences：The highest cartilage SNR and best CNR of cartilage versus bone cortex were both found in the 3D-FLASH（FA 20°） sequence,which was significantly different from the other three 3D-FLASH sequences.The satisfacory CNR of cartilage versus bone marrow,cartilage versus saline were found more in the 3D-FLASH（FA 20°） sequence.（2） C

关 键 词：腰椎间关节 软骨 磁共振成像 大体标本 

分 类 号：R445.2[医药卫生—影像医学与核医学]