肾移植及骨髓移植受者术后巨细胞病毒早早期抗原的检测  

Detection of cytomegalovirus immediate early antigen in kidney and bone marrow transplantation recipients

作　　者：吴国华[1] 罗灿峤[1] 赖英荣[1] 梁惠珍[1] 钟觉民[1] 梁英杰[1] 吴荣佩[2] 

机构地区：[1]中山大学附属第一医院病理科,广东省广州市510080 [2]中山大学附属第一医院泌尿外科,广东省广州市510080

出　　处：《中国组织工程研究与临床康复》2010年第44期8203-8206,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省自然科学基金(05300763)资助项目~~

摘　　要：背景:肾移植及骨髓移植后,随着免疫抑制剂的应用,潜伏的巨细胞病毒都将被激活。因此早期准确诊断巨细胞病毒感染具有重要的意义,及时而有效地控制巨细胞病毒感染的关键是在血液中迅速、定量地检测到巨细胞病毒的复制。目的:检测肾移植及骨髓移植受者术后的巨细胞病毒早早期抗原,探讨其临床诊断价值。方法:采用免疫组织化学方法对1215例肾移植及骨髓移植受者外周血白细胞巨细胞病毒活动性感染的早早期抗原进行检测。结果与结论:巨细胞病毒早早期抗原指数阳性492例(占40.0%),早早期抗原指数阴性723例(占60.0%),巨细胞病毒抗体IgG明显升高318例(占26.0%),巨细胞病毒抗体IgM90例(占7.0%)。巨细胞病毒抗原血症检测技术对于巨细胞病毒活动性感染的诊断具有高度的灵敏性和特异性,同时对巨细胞病毒的发生还具有重要的预测意义,是一种经济可行的诊断方法。BACKGROUND：With the application of immunosuppressive agents, latent cytomegalovirus （CMV） will be activated following kidney or bone marrow transplantation.Therefore, accurate diagnosis of early CMV infection has great significance.The key point of controlling CMV infection is detecting the CMV replication in blood rapidly and quantitatively.OBJECTIVE：To detect CMV immediate early antigen in renal and bone marrow transplantation recipients and to explore its clinical diagnostic value.METHODS：The immediate early antigens of peripheral blood leukocytes with active CMV infection in 1 215 cases receive kidney and bone marrow transplantation were detected by immunohistochemistry.RESULTS AND CONCLUSION：There were 492 cases （40.0%） with positive CMV immediate early antigen index and 723 cases with negative immediate early antigen index （60.0%）, CMV IgG antibodies were significantly increased in 318 cases （26.0%）, and CMV IgM antibody could be found in 90 cases （7.0%）.CMV antigenemia detection technology exhibits highly sensitivity and specificity in diagnosing active CMV infection and has important prognostic significance in detecting CMV incidence, thus, it is an economical and viable diagnostic method.

关 键 词：巨细胞病毒 肾移植 骨髓移植 早早期抗原 组织移植 

分 类 号：R617[医药卫生—外科学]