落新妇甙对肺移植大鼠外周血T细胞活化的影响  

Effects of astilbin on peripheral blood T cell activation in rats with lung transplantation

作　　者：林慧庆[1] 黄杰[1] 舒愠[1] 

机构地区：[1]武汉大学人民医院胸外科,湖北省武汉市430060

出　　处：《中国组织工程研究与临床康复》2010年第44期8225-8229,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:目前,对于排斥反应的研究主要是通过阻断T细胞活化来达到抑制排斥反应。而在众多体外试验中,落新妇甙及其类似物显示了典型的选择性免疫抑制特色。目的:观察落新妇甙对大鼠移植肺活化T细胞的影响及动态变化,探讨落新妇甙对大鼠移植肺活化T细胞的作用。方法:50只大鼠随机分为3组。其中10只不做移植,抽取外周血设为空白组。剩余40只大鼠随机均分供、受体,左侧单肺原位移植建模后再随机均分为2组:对照组肺移植后,用生理盐水灌胃;落新妇甙组移植后用落新妇甙灌胃。移植前、移植后第2,5天,分别用荧光标记抗体双染色结合流式细胞技术检测各阶段大鼠T细胞表达的活化抗原CD69、CD25和CD71的表达。结果与结论:移植后第2天,落新妇甙组检测的CD69、CD25和CD71抗原均显著高于空白组(P<0.01),但低于对照组差异有显著性意义(P<0.05)。移植后第5天,落新妇甙组CD69、CD25和CD71抗原基本降至正常水平,显著低于对照组(P<0.01)。结果提示落新妇甙能显著抑制急性排斥反应诱导的T细胞活化,对肺移植排斥反应有较强的抑制作用。BACKGROUND：Currently, rejection is inhibited by blocking T cell activation.Many in vitro experiments showed that, astilbin and their analogs exhibit typical selective immunosuppression.OBJECTIVES：To investigate the expression and implication of astilbin on peripheral blood T cell activation in rats with lung transplantation.METHODS：Fifty rats were randomly divided into 3 groups.Ten rats were taking peripheral blood as the blank group.The remained 40 rats were randomly assigned to donors and recipients.Model of rat left lung transplantation was set up.Twenty lung transplanted rats were divided in to 2 groups randomly：the control group, which was lavaged with normal saline, the experimental group was lavaged with astilbin.The expressions of T cell activation antigen CD69, CD25 and CD71 were determined by fluorescent-labeled antibody staining and flow cytometry （FCM） prior to and at 2, 5 days after transplantation.RESULTS AND CONCLUSION：The levels of CD69, CD25 and CD71 in the astilbin group were markedly higher at 2 days after lung transplantation than that of the blank group （P0.01）, but lower than the control group （P0.05）.At 5 days after lung transplantation, the levels of CD69, CD25 and CD71 in the astilbin group were declined into normal levels, which was notably lower than that of the control group （P0.01）.Astilbin significant suppresses acute rejection-induced T cell activation and shows strong inhibition effect on rejective reaction after lung transplantation.

关 键 词：肺移植 落新妇甙 T淋巴细胞活化 排斥反应 免疫抑制剂 

分 类 号：R617[医药卫生—外科学]