益肾通络方对膜性肾病大鼠肾组织中NF-κB、MMP-2表达的影响  

Effects of Yishentongluo decotion on the expression of NF-κB and MMP-2 on experimental membranous nephropathy

作　　者：檀金川[1] 田力铭[1] 张姝媛[2] 张林[3] 袁国栋[3] 

机构地区：[1]河北医科大学中医院,河北石家庄050011 [2]河北医科大学第一医院 [3]河北医科大学研究生院

出　　处：《中国老年学杂志》2010年第21期3111-3113,共3页Chinese Journal of Gerontology

基　　金：河北省科技厅科技攻关项目(No.08206115D)

摘　　要：目的观察益肾通络方对膜性肾病(MN)大鼠肾组织中核因子(NF)-κB、基质金属蛋白酶(MMP)-2的影响。方法将SD大鼠随机分为正常组、模型组、贝那普利组、中药组,各组按相应剂量灌胃,于第9周末观察24h尿蛋白定量、血清总蛋白(TP)、白蛋白(ALB);免疫荧光、电镜及免疫组化法检测各组大鼠NF-κB、MMP-2在肾组织的表达。结果与正常组比较,模型组大鼠出现大量蛋白尿,血清TP、ALB均明显降低,血清TC、TG均明显升高,肾脏病理出现损害,肾小球内NF-κB、MMP-2表达显著增强(P<0.05);与模型组相比较,各治疗组24h尿蛋白定量,总胆固醇(TC)及甘油三酯(TG)均明显降低,血清TP、ALB均明显升高,肾脏病理损害较轻,肾小球内NF-κB、MMP-2表达减少(P<0.05)。中药组与贝那普利组比较无明显差异(P>0.05)。结论益肾通络方可显著降低MN大鼠尿蛋白、提高血浆蛋白,改善血脂代谢,能够抑制NF-κB、MMP-2在肾组织中的表达,减轻足细胞融合,促进受损的肾小球基底膜(GBM)修复,从而减轻肾脏损伤,延缓肾脏慢性病理进展。Objective To observe the effects of Yishentongluo decotion on the expression of NF-κB and MMP-2 on experimental membranous nephropathy （MN）.Methods SD rats were randomly divided into control,model,benazepril and Chinese herb groups （10 rats each group）,and given with the medicine respectively.At the ninth week,24 h urine protein quant （UPro）,TP,ALB were determined,electron microscope and immunofluorescence methods were used to detect the expressions of NF-κB and MMP-2.Results Compared with those of normal group,the 24 h urinary protein quantitative analysis and the serum level of TC and TG of model group were sharply increased;the serum TP and ALB levels were significantly lower,nephritic injury incurred.The expression of NF-κB,MMP-2 in model group increased（P0.05）.Compared with those of model group,the 24 h urinary protein quantitative and the serum level of TC and TG of each treatment group were sharply decreased;the serum TP and ALB of were significantly higher;nephritic injury was lighter.The expression of NF-κB,MMP-2 in model group increased as compared to those of benazepril and Chinese herb groups（P0.05）.There was no markable difference between the Benazepril and Chinese herb groups（P0.05）.Conclusions Yishentongluo decotion can reduce rat＇s proteinuria,increase plasma protein,improve blood rheology to protect renal function.Yishentongluo decotion can depress NF-κB and MMP-2 expression in nephridial tissue.

关 键 词：益肾通络方 膜性肾病 核因子-κB(NF-κB) 基质金属蛋白酶-2(MMP-2) 

分 类 号：R692[医药卫生—泌尿科学]