降钙素对关节软骨细胞中β连环蛋白和分泌型frizzled相关蛋白1的影响  

Effect of calcitonin on beta-catenin and secreted frizzled-related protein 1 in articular cartilages

作　　者：张尹娜[1] 王文雅[1] 张柳[2] 胡宏宇[2] 李彬[2] 王哲彦[1] 

机构地区：[1]华北煤炭医学院病理教研室,河北省唐山市063000 [2]华北煤炭医学院附属医院骨外科,河北省唐山市063000

出　　处：《中国组织工程研究与临床康复》2010年第41期7601-7604,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：河北省科技领军人才创新基金项目(06547008D-8)~~

摘　　要：背景:研究表明降钙素具有抗骨吸收作用,对软骨有保护作用,但其具体机制尚不明确。目的:探讨降钙素是否通过Wnt/β信号通路对关节软骨细胞起保护作用。方法:分离培养雄性日本大耳白兔关节软骨细胞,随机分为对照组,白细胞介素1β组和降钙素组,后两组细胞于传代后的第2天加入白细胞介素1β(1×10-5g/L)模拟骨关节炎发生,降钙素组第4天换成含有降钙素的完全培养基。第6天采用免疫细胞化学法检测β连环蛋白的表达,以及实时荧光定量PCR法检测β连环蛋白与分泌型frizzled相关蛋白1mRNA的表达。结果与结论:降钙素组β连环蛋白和mRNA的表达显著低于白细胞介素1β组(P<0.05);白细胞介素1β组β连环蛋白和mRNA的表达显著高于对照组(P<0.05)。降钙素组分泌型frizzled相关蛋白1的mRNA表达显著高于白细胞介素1β组(P<0.05),白细胞介素1β组分泌型frizzled相关蛋白1mRNA表达显著低于对照组(P<0.05)。这些结果说明降钙素能抑制关节软骨细胞的进一步损伤,可能是通过Wnt/β连环蛋白信号通路对关节软骨细胞起保护作用。BACKGROUND： Researches have reported the protection function of calcitonin for chondrocytes via preventing bone resorption, but the mechanism remains unclear. OBJECTIVE： To assess whether calcitonin exerts a protective action through the Wnt signal pathway in chondrocytes. METHODS： The chondrocytes were separated from Japanese male rabbits and randomly divided into the control, interleukin 1 β and calcitonin groups. In the later two groups, interleukin 1 β （1×10-5 g/L） was added at 2 days after passage to simulate osteoarthritis, and then DMEM containing calcitonin was used to culture the cells in the calcitonin group at 4 days after passage. The expression of β-catenin protein was detected by immunocytochemistry, and the mRNA of β-catenin and secreted frizzled-related protein 1 （sFRP-1） were determined by real-time PCR. RESULTS AND CONCLUSION： The expression of β-catenin and its mRNA in the calcitonin group was obviously lower than that of the interleukin 1 β group （P0.05）. Compared with the control group, the expression of β-catenin and its mRNA in the interleukin 1 β group appeared to be greater （P0.05）. The expression of sFRP-1 mRNA was notably higher in the calcitonin group than that of the interleukin 1 β group （P0.05）, which was lower than the control group （P0.05）. The results demonstrated that calcitonin can inhibit the further damage of articular cartilage, which may play its protective role via Wnt/β-catenin signal pathway.

关 键 词：降钙素 骨性关节炎 白细胞介素1β Β连环蛋白 分泌型frizzled相关蛋白1 

分 类 号：R318[医药卫生—生物医学工程]