人关节软骨源性海绵的制备和评估  

Preparation and evaluation of human articular cartilage-derived sponge

作　　者：张建党[1] 卢世璧[2] 袁玫[2] 黄靖香[2] 孙明学[2] 赵斌[2] 

机构地区：[1]解放军306医院骨科,北京市100101 [2]解放军总医院骨科研究所,北京市100853

出　　处：《中国组织工程研究与临床康复》2010年第41期7617-7620,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:关节软骨损伤后的修复是目前骨科领域的难点之一,组织工程的发展应用为关节软骨损伤的修复展示了诱人前景,然而理想的支架却是组织工程软骨的成功构建的关键。目的:制备人关节软骨源性海绵,为组织工程软骨提供合适的新型支架。方法:应用人关节软骨制备人关节软骨源性海绵。应用扫描电镜观察对人关节软骨源性海绵进行大体和微观观察,观察人关节软骨源性海绵在紫外线照射前后的抗离散情况,并采用压汞法测定其孔径和孔隙率。结果与结论:人关节软骨源性海绵的抗离散性能良好,孔径大小在100～200μm,孔隙分布均匀,孔隙率为92%。结果表明人关节软骨源性海绵具有良好的结构和孔隙率,可作为软骨组织工程支架。BACKGROUND： The repair of articular cartilage remains one of the most challenging topics in orthopedic research. Tissue engineering may be a promising way to restore functional hyaline cartilage of joints. Scaffold is one of the main elements in tissue engineering. OBJECTIVE： To prepare human articular cartilage-derived sponge so as to provide a novel cartilage tissue engineering scaffold. METHODS： Human articular cartilage-derived sponge was prepared using human articular cartilage. Human articular cartilage derived sponge was investigated by scanning electron microscope. Its porosity was detected by mercury intrusion porosimetry, and average pore diameter was determined by using scanning electron microscope image analysis system. RESULTS AND CONCLUSION： Human articular cartilage-derived sponge presented with good anti-discreteness, with 100-200 μm pore diameter and 92% porosity. Human articular cartilage derived-sponge possessed the characteristics that afford it as a potential scaffold for cartilage tissue engineering.

关 键 词：关节软骨 海绵 支架 组织工程 胰蛋白酶 

分 类 号：R318[医药卫生—生物医学工程]