功能性矫治器引导下颌前伸后咀嚼肌中基质金属蛋白酶抑制因子及Ⅳ型胶原参与咀嚼肌的适应性变化  

Expression of tissue inhibitor of matrix metalloproteinases and type Ⅳ collagen in growing rat masticatory muscle after functional mandibular protrusion

作　　者：孙慧玲[1] 周洪[2] 邹敏[2] 

机构地区：[1]西安交通大学医学院附属口腔医院,口腔医学研究中心,陕西省西安市710004 [2]西安交通大学医学院附属口腔医院,正畸科,陕西省西安市710004

出　　处：《中国组织工程研究与临床康复》2010年第41期7661-7664,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：陕西省科技攻关项目(2006k11-G6(2)),功能矫形前伸下颌后大鼠咀嚼肌TIMPs及IV胶原表达变化研究~~

摘　　要：背景:功能性矫治器引导大鼠下颌矫形治疗中,咀嚼肌也会发生适应性变化和改建,基质金属蛋白酶参与其中变化。目的:观察功能性矫治器引导大鼠下颌前伸后咀嚼肌中基质金属蛋白酶抑制因子及其底物作用蛋白Ⅳ型胶原表达。方法:4周龄健康雄性SD大鼠随机分为实验组和对照组。实验组每日白天戴矫治器10～12h。采用免疫组织化学方法观察戴矫治器后3,7,14,21d大鼠二腹肌前腹中基质金属蛋白酶抑制因子1,2及Ⅳ型胶原的表达。结果与结论:实验组和对照组大鼠二腹肌前腹中基质金属蛋白酶抑制因子1,2及Ⅳ型胶原均有表达。但实验组IV型胶原、基质金属蛋白酶抑制因子1,2表达明显增强,表明功能性矫治器引导大鼠下颌前伸后咀嚼肌中基质金属蛋白酶抑制因子1,2及Ⅳ型胶原参与了咀嚼肌的适应性变化。BACKGROUND： Rat masticatory muscle presented with adaptability and remodeling during functional mandibular protrusion, and matrix metalloproteinases was involved in this process. OBJECTIVE： To investigate tissue inhibitor of matrix metalloproteinases （TIMPs） and collagen type Ⅳ changes in growing rat masticatory muscle after functional mandibular protrusion. METHODS： Healthy male Sprague Dawley rats, aged 4 weeks, were randomly divided into the experimental and control groups. Rats in the experimental group wear appliance 10-12 hours per day. The expressions of TIMP-1, TIMP-2 and type Ⅳ collagen in growing rat masticatory muscle were measured at 3, 7, 14 and 21 days by immunohistochemistry. RESULTS AND CONCLUSION： TIMP-1, TIMP-2 and type Ⅳ collagen were expressed in digastric muscle in all groups. However, compared with the control group, the expression of TIMP-1, TIMP-2 and type Ⅳ collagen was enhanced in the experimental group, which suggested that, TIMP-1, TIMP-2 and collagen type Ⅳ involve changes in growing rat masticatory muscle after functional mandibular protrusion.

关 键 词：咀嚼肌 基质金属蛋白酶抑制因子1 基质金属蛋白酶抑制因子2 Ⅳ型胶原 下颌前伸 

分 类 号：R318[医药卫生—生物医学工程]