microRNA在肌肉发育和疾病中的作用  

Role of microRNA in muscle development and disease

作　　者：李东颖[1] 史斌[1] 严春辉[1] 

机构地区：[1]西安科技大学体育部,陕西省西安市710054

出　　处：《中国组织工程研究与临床康复》2010年第41期7743-7747,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:microRNA研究在肿瘤、遗传及细胞增殖、分化等领域已取得显著的成就,而在肌肉发育及其相关疾病中的研究刚刚起步。目的:综述国内外关于microRNA的研究进展及其与肌肉发育和疾病的密切关系。方法:应用计算机检索中文CNKI数据库、超星数字图书,检索关键词为microRNA,肌肉和心血管疾病等;英文数据库为PubMed和ISI web of knowledge,检索关键词为"microRNA,muscle,cardiovascular disease"等;此外还手工查阅多部相关专著。纳入与microRNA的位置、结构与功能,microRNA与骨骼肌和心肌发育及肌肉疾病相关的37篇文献进行分析。结果与结论:microRNA是一种高度保守的小的非编码RNAs,通过与靶基因mRNA分子3’端未翻译区域(3’-untranslatedregion,3’UTR)特异性结合,负性调节转录后的基因表达。遗传研究表明,microRNAs是肌肉发育和功能所必需的,其参与了肌肉发育及其肌肉相关疾病包括心肌肥厚、心律失常和肌营养不良症等过程,这说明microRNA与肌肉发育和疾病密切相关,怎样通过调控microRNA来预防和治疗肌肉疾病将成为后续研究的重点。BACKGROUND： Study of microRNA has made great progress in the fields of tumor, heredity, cell proliferation and cell differentiation. However, it is still at the beginning in the research of muscle development and related disease. OBJECTIVE： To review research advances of microRNAs and its relationship with muscle development and disease. METHODS： A computer-based online search of CNKI （http：//acad.cnki.net/） and SSReader （http：//edu.sslibrary.com） was performed with Key words of ＂microRNA, muscle and cardiovascular disease＂ in Chinese; Pubmed （http：//www.ncbi.nlm.nih.gov/） and ISI Web of Knowledge （http：//apps.isiknowledge.com/） were searched with key words of ＂microRNA, muscle and cardiovascular disease＂ in English. In addition, related articles were manually searched. A total of 37 documents regarding position, structure, function of microRNA; microRNA and muscle development; microRNA and muscle-related disease were included in the final analysis. RESULTS AND CONCLUSION： microRNAs are a class of highly conserved small noncoding RNAs that can specially bind to 3＇-untranslated region of the target mRNA and negatively regulate gene expression post-transcriptionally. Genetic studies have demonstrated that miRNAs are required for both proper muscle development and function, and as regulators of muscle development, as well as in muscle-related disease processes, including cardiac hypertrophy, arrhythmia, and muscular dystrophy. These suggest that microRNA and muscle development and disease are closely related. It has been confirmed that microRNA is highly correlated with muscle development and disease. However, how to regulate microRNA for the prevention and treatment of muscle diseases will be the focus of future study.

关 键 词：骨骼肌 心肌 MICRORNA 肌肉发育 心血管疾病 肌肉肌腱组织工程 

分 类 号：R318[医药卫生—生物医学工程]