5/6肾切除大鼠模型尿纤溶酶原激活物活性的动态观察  

Dynamic observation of urinary plasminogen activator excretion in 5/6 nephrectomized rats

作　　者：徐启河[1,2] 陈香美[1,2] 张颖[1,2] 师锁柱[1,2] 文载律[1,2] 

机构地区：[1]解放军总医院肾科 [2]解放军总医院病理科

出　　处：《军医进修学院学报》1999年第2期106-108,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金

摘　　要：目的：了解５／６肾切除大鼠尿纤溶酶原激活物（ＰＡ）排泄量的动态变化。方法：５／６肾切除术后不同时间点分别检测尿ＰＡ活性以及血、尿生化指标，并进行肾脏光镜、电镜检查。结果：术后２周血肌酐显著升高但肾脏病理改变轻微，多数大鼠尿ＰＡ排泄量仍能维持正常水平。术后４周以肾小球肥大和系膜增殖改变为特征，血肌酐正常；部分大鼠尿ＰＡ排泄量减少，也有部分大鼠尿ＰＡ排泄量增加。术后６～８周肾功能失代偿，尿蛋白大量排出，肾脏病理以肾小球局灶节段硬化、新月体形成、纤维素样渗出，以及肾小管－间质病变为特征。此期尿ＰＡ排泄量逐渐增高，８周时２４ｈ尿ＰＡ排泄量显著高于假手术组。结论：５／６肾切除术后，残余肾脏排泄ＰＡ逐渐增加。Objective:to observe the urinary plasminogen activator (PA) excretion in 5/6 nephrectomized rats. Methods:Light and electronic microscopic examination of the kidney were carried out at different time points after 5/6 nephrectomy, and urinary PA excretion rate as well as blood and urine biochemical parameters were dynamically observed.Results: Two weeks after 5/6 nephrectomy, acute renal failure induced by renal mass loss occurred, but renal pathologic changes was minimal. In this stage, the urinary PA activity of some rats decreased, but that of the others maintained normal level despite the renal mass loss. Four weeks after 5/6 nephrectomy, glomerular hypertrophy and mesangial proliferation became the characterized pathological changes,and the serum creatinine was nearly normal. In this stage, urinary PA activity decreased in some, and normalized or even increased in the others. Six to eight weeks after 5/6 nephrectomy, chronic renal insufficiency and heavey proteinuria occurred, renal pathology was characterized by focal and segmental glomerulosclerosis, as well as progressive tubulo interstitial changes. In this stage, urinary PA excretion increased gradually, and total urinary PA output increased significantly at 8 weeks after 5/6 nephrectomy comparing with the sham operated group, P <0 05.Conclusion:5/6 nephrectomy might trigger the compensatory increase of PA excretion in urine.

关 键 词：肾切除 纤溶酶原激活物 病理学 肾切除 

分 类 号：R699.202[医药卫生—泌尿科学]